Dynamic Expression Of VEGF And EPO In Rat Corneal Neovascularization Tissues After Alkali Burn

Background:The course of corneal repair after alkali burn is often accompanied by the occurrence of neovascularization. Corneal vascularization which often leads to loss of corneal transparency is not only an important cause of blindness,but is also a risk factor of rejection after keratoplasty. It has been found that many cytokines involved in the formation of angiogenesis which is a complex biological procedure.Purpose:To evaluate the relationship of vascular endothelial growth factor(VEGF) and erythropoietin(EPO) between expression and aging in the formation of angiogenesis by using the rat corneal alkali burn model,thereafter provides a theoretical basis for the clinical research and treatment of corneal alkali burn-induced corneal neovascularizationMethod:The rat alkali burn-induced corneal neovascularization models were made.The new blood vessels in the injured corneas of rat were examined using slit lamp.The expression of VEGF and EPO proteins were detected using immunohistochemical assay at 3d,5d,14d after injury.The expression levels of VEGF mRNA and EPO mRNA were quantified with reverse transcription polymerase chain reaction(RT-PCR). Result:The expression of VEGF,EPO protein and mRNA were minimally detected in the normal corneas of rat,and increased 3d after alkali burn,which reached the peak 5d,then decreased 14d after the injury.Conclusion:1)After corneal alkali burn,the expression of VEGF mRNA and protein upregulate in the forming process of corneal neovascularization.2) After corneal alkali burn,the expression of EPO mRNA and protein upregulate in the forming process of corneal neovascularization.3) After corneal alkali burn,there is a time relevance between the expression of VEGF and EPO and corneal neovascularization.