Synthesis And Anti-tumor Mechanism In Human Gastric Carcinoma Cell Of Furanoside Of Artemisia Sacrorum

Objective: A large amount of furanoside of Artemisia sacrorum was synthesized by chemical method.The molecular mechanism of apoptosis and autophagy in human gastric cancer cell(MKN-45)induced by Artemisia furonidin in vitro is studied,and the molecular basis of the relationship between apoptosis and autophagy under the induction of furfurone glycoside is discussed.Methods Anisaldehyde was used as raw material to synthesize furanoside of Artemisia sacrorum by affinity addition,electrophilic substitution and dehydration,and the structural formula was identified by NMR method.The effect of furanoside of Artemisia sacrorum on the proliferation of MKN-45 was observed by MTT method.After the treatment group(control group,700 mol/L group,800 mol/L group),the total proteins were extracted at 24 h after administration,autophagy related proteins(LC3B,Beclin1)and apoptosis related proteins(Bax,Bcl-2,Caspase-3)expression level was detected by Western Blot.Results A large number of furanoside of Artemisia sacrorum were synthesized by chemical synthesis,with a yield of 53%.After the analysis of 1H-NMR,13C-NMR,DEPT,HSQC,and HMBC atlas,the structure was determined to be 6-hydroxyl-4’-methoxyArtemisia furarone-4-O-beta-D-glucoside.The concentration of furanoside of Artemisia vestita in the range of 400-1000 mol/L was 24h-36 h,and the proliferation of MKN-45 cells was obviously inhibited(P<0.01).Western Blot results showed that furanoside of Artemisia sacrorum could induce apoptosis and autophagy in MKN-45 cells,that is,it can increase the expression level of BAX(P<0.01),Caspase-3(P<0.01),autophagy protein Beclin 1(P<0.01)and LC3II/LC3I(P<0.01),and reduce the expression level of inhibitor of apoptosis protein BCL-2(P<0.01).Conclusion: The synthetic process of Furanoside of Artemisia sacrorum is simple,the reaction conditions are mild,the raw material is easy to be obtained,and it is easy to be prepared on a large scale.Furanoside of Artemisia sacrorum showed anti-tumor activity and inhibited the proliferation of MKN-45 cells and induced apoptosis and autophagy of MKN-45 cells.