Study Of Active Parts Of Artemisia Sacrorum Ledeb. On The Anti-Hepatic Injury

Objective: To study the protective effect of macroporous resin elutes of Artemisia sacrorum Ledeb. extract on the acute hepatic injury in mice and investigate the inhibitive effects of macroporous adsorbing resin elute of Artemisia sacrorum Ledeb. extract on proliferation of HepG-2 cells.Method: 1) Water extract of Artemisia sacrorum Ledeb. was loaded on D-101 macroporous resin and eluted by water, 50% ethanol and 95% ethanol respectively, thereby different elutes were obtained. 2) The mouse models of acute hepatic injury were induced by carbon tetrchloride (CCl₄) and acetaminophen (APAP) respectively, and the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of the mice, and the liver levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of the mice were measured, and the pathological examination of liver was performed. 3) MTS assay was used to examine the effect of macroporous resin elutes of Artemisia sacrorum Ledeb. extract on the proliferation of HepG-2 cell.Results: 1) The supernatant of the 50% ethanol elute of Artemisia sacrorum Ledeb. extract greatly decreased ALT and AST levels in serum and increased SOD activity in liver tissue and decreased MDA level in liver compared with CCl₄ - induced model group. 2) Both the precipitation and the supernatant of 50% ethanol elute of Artemisia sacrorum Ledeb. extract decreased ALT and AST levels in serum and increased SOD activity in liver tissue and decreased MDA level in liver compared with the APAP-induced model group. 3) The liver tissue pathology also showed that both the precipitation and the supernatant of the 50% ethanol elute of Artemisia sacrorum Ledeb. extract could alleviate liver tissue injury. 4) The 95% ethanol elute of Artemisia sacrorum Ledeb. extract inhibited the proliferation of human hepatocellular carcinoma cell line (HepG-2) cell and the effects were in a time- and concentration-dependent manner.Conclusions: 1) The 50% ethanol elute of Artemisia sacrorum Ledeb. extract has protective effects against acute hepatic injury. 2) The 95% ethanol elute of Artemisia sacrorum Ledeb. extract inhibits the proliferation of HepG-2 cells effectively.