Research On Quality Standard Of Artemisia Sacrorum Of Korean Traditional Crude Herbs In Yanbian

Objective To evaluate the quality of Artemisia sacrorum in Yanbian with the parameter of quality evaluation, To explore the Microscopiic Identification on the traditional korea medicinal plant, To establish chromatographic fingerprint of Artemisia sacrorum by using HPLC and TLC for identifying and evaluating its quality,and to set up the quantiative method with HPLC. to redeem the the blank of drug standard of Jilin at 1977 and promoting the quality standard of Artemisia sacrorum to provid the science data.Methods 1.According to the addendaumⅨH、ⅨK、ⅩA、ⅧH andⅧJ in The Pharmacopoeia of PRC at 2011, to assay the content of water,ash,extractum,heavy metals(pb2+) in Artemisia sacrorum.2. The paraffin section technology was used to observe the microscopic identifications of stems and leaves of Artemisia sacrorum. The slide with chloral hydrate was used to observe the microscopic identifications of the powder of stems and leaves.3.To setup the chromatographic fingerprint of Artemisia sacrorum by using HPLC and TLC,to provid the data for the quality control and variety identification,the RP-HPLC with an analytical column Diamonsil-C18(250 mmx4.6mm,5μm) was used. A mobile phase consisted of methanol-0.4% phosphoric acid water solution (v/v) with linear gradient elution was used as mobile phase at the flow rate of 1.0 mL-min-1, The column temperature was 35℃and detection wavelength was at 360 nm.4.To take the maximum administration dosage and modified Kaber method to process the safety evaluation. Results 1. The standard parameter of corresp in Artemisia sacrorum:the content of water is less than 8.00%,the total ash is less than 7.0%,the acid-insoluble ash is less than 1.0%,the water-soluble extractivesis less than19.19%, the extractum of diluted Alcohol is less than 20.00%,the heavy metals(pb2+)is less thanlOppm and the arsenic salt is less than 2ppm.2 The microscopic identifications of stems and leaves and their powder:Phloem has a large number of fibers and the pith was wide in the transection of stem.And one palisade cell in the transection of the leaves.Meanwhile,cork cell,nonglandular hairs and spiral vessels,borderedpit catheter cluld be found in powder;To setup the chromatographic fingerprint of Artemisia sacrorum by using TLC,8 spottedness have the better reproducibility;in theidentification of qualitation by TLC,at 254nm,the Rf of scopoletin、quercetol、luteolo is 0.75、0.25、0.185.3.According to the result for the nine different groups of Artemisia sacrorum in Yanbian, choose thel6 peaks of the stabile retention time and peak area scaling the chemical constituent of chromatographic fingerprint of Artemisia sacrorum,it has the better systematicness, mark, repetitiveness. through the evaluation of semblance,the semblance of the eight towns of Artemisia sacrorum is more than 0.90 which has the better dependablity.4.To compare the result of the contet of HPLC,the different Artemisia sacrorum in Yanbian has the disparation,5.The maximum administration dosage was 15 g of Artemisia sacrorum powde given to mice per kilogram,equivalent to men’s (60kg) daily administration dosage by102-153 times; median lethal dose of the extract of Artemisia sacrorum was 104.40g/kg,95% confidence interval was 95.46-114.18g/kg, it is safe which it is in the dose range of general all purpose. Conclusion The microscopic identifications of Artemisia sacrorum and the specificity of TLC; the chromatographic fingerprint of Artemisia sacrorum by using HPLC and TLC;Simultaneous determination of Scopoleti、Quercetol and Luteolol from Artemisia sacrorum by RP-HPLC;the extract of Artemisia sacrorum and Artemisia sacrorum powder are to process the safety evaluation,the research is to redeem the the blank of drug standard of Jilin at 1977 and promoting the quality standard of Artemisia sacrorum to provid the science data.