The Effect Of Help Liver Yang Disappear Phlegm And Refreshing On Depression Rats Wnt/?-catenin Signaling Pathway

Research purpose:This study isolated for combination of chronic stress depression model rats as the research object,from the interpretation of indicators,such as ethology and hippocampal neurons morphology change helps liver Yang phlegm and begin to understand the method on the curative effect mechanism of depression model rats,through the RT-qPCR detection,GSK DVL-1-3 beta,Axin,APC,beta catenin mRNA expression,helps liver Yang phlegm and begin to understand the method of antidepressant effect and its mechanism of action.Research methods:Theoretical research part:based on the "The brain is the home of the primordial spirit","the man's primordial spirit,founded on the kidneys,and germination in liver" theory,put forward from bone related treatment of depression as the starting point,say a lack of liver Yang,fu,phlegm turbidity intruders,alongwith the brain god as the main pathogenesis of depression,support the Help Liver Yang Disappear Phlegm and Refreshing as the basic law,Treat depression with ChaiGuiWenDanDingZhiTang.Experimental study:60 Wistar rats were randomly divided into 6 groups,respectively.Model group;The high-dose group of ChaiGuiWenDanDingZhiTang;The dosage groups in the ChaiGuiWenDanDingZhiTang;The low-dose group of ChaiGuiWenDanDingZhiTang;Fluoxetine group.10 for each group.Through solitary have combined with chronic mild unpredictability stimulus preparation of depression model rats,rats in experiment 1,21 days to observe the general situation,measure the body weight of rats,Open box experiments(Open-field-test)and sucrose preference experiments.In experiment,21 days after the behavior observation,the rat hippocampal samples,by HE staining to observe rat hippocampal morphological structure changes and by real-time fluorescent quantitative PCR detection rat hippocampus,GSK DVL-1-3 beta,Axin,APC,beta-catenin mRNA expression,to explore helps liver Yang phlegm and begin to understand the method of depression model rats Wnt/beta-catenin signaling pathway.Research results:1,through the lonely have chronic mild unpredictability stimulation method building after 21 days,blank group rats weight and model groups had significant difference(P<0.01),and high-dose group of Chinese medicine weight fluoxetine group was obviously higher than that of model group(P<0.01),Open box experiment(Open-field-test)show that fluoxetine group and high dose group of Chinese medicine horizontal movement and vertical movement of rats scores were significantly higher than that of model group(P<0.01),sucrose preference experiments suggest fluoxetine group and high dose group of Chinese medicine in the rat sugar water intake significantly higher than the model group(P<0.01).2.HE staining suggested that the rat hippocampal neurons in the high-dose group and fluoxetine group were higher than the model group(P<0.05).3.RT-qPCR shows that compared with the blank group,model group rats decreased DVL-1 mRNA expression,GSK-3 beta,Axin,APCmRNA expression quantity to increase,beta catenin mRNA expression decreased,while the western medicine group and high dose group suggests that DVL increased 1 mRNA expression and GSK-3 beta,Axin,APCmRNA expression decreased,beta catenin increased mRNA expression.Conclusions:1,help the liver Yang phlegm begin to understand the method of weight loss of depression model rats,decreased activity could improve the behavior,the depression model rats hippocampus CAl area neurons has certain protective effect.2,under stress,the rats Wnt/beta-catenin signaling pathways suppressed,hippocampal neurons is damaged,and helps the liver Yang phlegm can begin to understand method activation of Wnt/beta-catenin signaling pathway,promote the occurrence of hippocampal neurons,protect hippocampal neurons,give play to the role of antidepressants.