| AimTo evaluate the role of chitosan in Calcium alginate Gauze(CG)for wound healing in vitro and in vivo.MethodsHigh and low molecular weight chitosan were incorporated into CG to build a functional CG,Chitosan-Calcium alginate Gauze(CCG).Characteristics of CCG,including cytotoxicity,antibacterial activity and moisture retention were tested.Evaluation of the wound-healing potential of CCG was performed in Sprague-Dawley rats with a full-thickness excisional wound.At days 3,7 and 14 after operation,wound healing was monitored through macroscopic and histological analysis.ResultsCompared with CG,CCG shows better antibacterial property and moisture-retention ability with little cytotoxicity to NIH3T3 cells.Wounds dressed with CCG showed significant increase in wound contraction rate,shorter epithelialization time(17 ± 1.80 day vs 21 ±1.45 day,p<0.05).CCG also effectively promotes faster angiogenesis and horizontally-oriented collagen.ConclusionChitosan in CCG offers good antibacterial and moist environment and promotes wound healing evidently.These results demonstrate the potential value of CCG in healing properties and clinical applicationg. |
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