Study On The Association Between TCF7L2 Gene Polymorphisms And Gestational Diabetes Mellitus

Objective To explore the relationgship between the polymorphism of the transcription factor 7-like 2(TCF7L2)gene(rs7903146C/T and rs12255372 G/T)and genetic susceptibility of gestational diabetes mellitus(GDM)in Chinese Han population of Fujian Province,and to analyze the related clinical characters of GDM.Methods By adoping the method of 1:1 frequency matched case-control study,information was collected retrospectively from 100 pregnant women with GDM and 100 healthy pregnant women delivered in Fujian Maternity and Children Health Hospital during July 2014 to December 2014.High Performance Liquid Chromatography was used to detect Hb A1C;single nucleotide polymorphism shot(Sna Pshot)was used to analyze the variant of TCF7L2 rs7903146,rs12255372 and whether the two related phenotypes would confer remarkable risk for GDM was estimated by statistical methods.The clinical indexes and glucose metabolism related indicators including age,family history,BMI,FPG,Hb A1 C and blood lipids were measured.Results1.Gestational age,BMI,FPG and Hb A1 C between the two groups were statistically significant(P<0.05),and there was no statistical difference between the two groups in family history of diabetes,blood lipids,gain weight,and neonatal weight(P >0.05).2.The genotype frequencies of TCF7L2 gene rs7903146 position were 96%and 4% respectively for CC and CT genotypes in the control group;92% and 8%respectively in the case group.TT genotype was no found in both groups.The allele frequencies were 98% and 2% in the control group,and 96% and 4% in the case group for C,T alleles respectively.No statistical difference was found in the diversities of genotype and allelic frequencies between both groups(P >0.05).3.The genotype frequencies of TCF7L2 gene rs12255372 position in the control group were 100% and 0% respectively for GG,GT genotypes;98% and 2%respectively in the case group.TT genotype was no found in both groups.The allele frequencies were 100% and 0% for G,T alleles in the control group;99% and1% respectively in the case group.There was no statistical difference in the diversities of genotype and allelic frequencies between the two groups(P >0.05).4.The level of OGTT1 h BG was higher in CT genotype of 7903146 position than that in CC genotype(P <0.05)in the case group.There was no significant difference between two groups in other parameters(P >0.05).There was only one kind of genotype in rs12255372,so it could not be statistical analyzed.5.Logistic regression analysis showed that age,BMI,and FPG were risk factors for GDM,whereas Hb A1 C was a protective factor.The risk of GDM in pregnant women with Hb A1 C <5.2% was 0.116 fold of those with Hb A1 C >5.5%,and 0.279 fold of those with Hb A1 C between 5.2% to 5.5%.Conclusions1.There were polymorphisms of the TCF7L2 gene rs7903146 C/T,rs12255376G/T in the tested cohorts and T allele was in low frequency distribution.2.The genetic characteristics of rs7903146,rs12255372 in TCF7L2 gene were irrelenvant to GDM.3.Age,BMI and FPG were risk factors for GDM,whereas Hb A1 C was a protective factor for GDM.The lower Hb A1 C was,the stronger protective effection it had.