Research On The Genetic Susceptibility Correlationship Of Rs7903146rs290487、rs11196205、rs12255372Polymorphism In The TCF7L2Gene With Gestational Diabetes Mellitus

ObjestiveTo investigate the genetic susceptibility correlationship of transcription factor7-like2(TCF7L2) gene rs7903146C/T、rs290487C/T、rs11196205G/C、rs12255372G/T polymorphism and Chinese Han population in Jiangxi Province with gestationaldiabetes mellitus．MethodProcess of object：Information was collected retrospectively from100Pregnantwomen with gestational diabetes Mellitus had been diagnosed and100healthyPregnant women who delivered at jiangxi Women and Children Hospital and HealthInstitute during January2010and June2013.Experimental method：By adopting the method of case-control study,The clinicalindicators and glucose metabolism related indicators were measured．Genomic DNAwas extracted from peripheral blood with Blood genomic DNA extraction kitextraction method.Allele-specific PCR was used to analyze the SNPs of TCF7L2rs7903146、rs290487、rs11196205、rs12255372.Statistic analysisTCF7L2gene rs7903146, rs290487, rs11196205, rs12255372genotypedistributions were the Hardy-Weinberg equilibrium (P>0.05).Statistical analysis wasconducted with Pearson chi-square test and t-test (SPSS17.0software) and P <0.05was considered statistically significant.ogistic regression analysis was an independentrisk factor binomial classification variables to P <0.05was considered statisticallysignificant.Results1The distribution frequencies of genotypes CC，CT and TT of TCF7L2geners7903146position in Jiangxi pregnancies were47.5%、37.0%and15.5%；Thefrequency of CC,CT and TT genotype of TCF7L2rs290487position were14.0%、35.0%and51.0%；And the frequency of GG and CC genotype of TCF7L2rs11196205position were99,5%、0.5%；And the frequency of GG genotype of TCF7L2rs12255372position were100%.2TCF7L2gene rs7903146、rs290487、rs11196205、rs12255372polymorphismwas consistent with Hardy-Weinberg equilibrium law. the difference of distributiongenotype distribution and C/T allele frequency of rs7903146between the two groupswas statistically significan（tP＜0.05）.Compared with C allele carriers，TT genotypecarriers had a4.195fold increased risk of GDM (95％CI1.715～10.266);P=0.002．No differences of genotype and allelic frequencies at rs290487、rs11196205and rs12255372were observed between the cases and the contrlis.3BMI, FPG, FINS, HOMA-IR between the case and control groups werestatistically significant (P <0.05).4Logistic regression analysis showed after adjusting for age,gestationalage,BMI, FPG, FINS, and neonatal birth weight, pregnant women with TT genotypecarriers of rs7903146were more prone to hyperglycemia compared with the C allelecarriers(OR2.77，95％CI1.027～7.474);P=0.044.BMI, FPG and FINS areindependent risk factors for GDM.Conclusions：The polymorphisms of rs7903146in TCF7L2gene may be associated withgestational diabetes mellitus in Jiangxi Han population; BMI, FPG, and FINS areindependent risk factors for GDM.