Application Of HPV And P16,Ki-67 In Early Diagnosis Of Cervical Lesions In Tibetan

Objective:Through the investigation of 270 cases of Tibetan women HPV subtypes detection and combined detection of biological markers of P16,the positive expression level of Ki-67,a preliminary analysis of 270 cases of cervical lesions of Tibetan women at all levels and the detection rate of HPV 15 HR-HPV ranking,to investigate the biological markers of P16,the positive expression of the role of Ki-67 in 270 cases of Tibetan women cervical precancerous lesion classification.Methods : Access to the pathology department of Affiliated Hospital of Qinghai University from 2010 year 03 months-2016 year 11 month had applied HPV genotyping detection of HPV Tibetan women with cervical lesions in patients with pathological paraffin blocks of 270 cases,including cervical chronic inflammation group,CINI group,CINII group,CINIII group at all levels of disease were 55 cases in each block cases of cervical squamous cell carcinoma,50 cases of paraffin blocks,all cases were recorded HPV.Line P16 and Ki-67 detection,according to the P16 and Ki-67 expression score to make the corresponding classification of cervical lesions.Results:1,In the 270 cases of Tibetan women,the total detection rate of HPV was 28.8%,the detection of single HR-HPV infection ratio was 92.30%;the detection of type two infection ratio of HPV was 3.80%;the detection of type three HPV mixed infection ratio is 1.28%;the detection of single LR-HPV infection ratio is 2.56%.The detection of the top five HR-HPV is HPV16,HPV58,HPV53,HPV39,HPV52.2,In this group of cases,P16 expression was negative in all the patients with chronic inflammation(100%).The positive rate of CINI group was 85.45%,the positive rate of CINII group was 100%,and the positive rate of CINIII group was100%.The positive expression rate of P16 in cervical squamous cell carcinoma group was 100%.3,In this group of cases,cervical chronic inflammation group of 55 cases,Ki-67 were negative expression,which accounted for negative expression of 56.36%,1+accounted for 43.63%.The positive rate of CINI was 69.09%,the positive rate of CIN II was92.72%,and the positive rate of CINIII was 100%.The expression rate of Ki-67 in cervical squamous cell carcinoma group was 100%.4,Combined with the expression of P16 and Ki-67,a new pathological grading of cervical lesions was made.After the diagnosis,1 cases of HE diagnosed pathologically were classified into chronic inflammation group,and 4 cases of CIN II were classified into LSIL group.51 cases of CIN II were classified as HSIL group.Conclusion:1,The study of Tibetan women,the detection rate of HPV increase with the increase of cervical lesions and high,mainly in the detection of single HR-HPV infection.HR-HPV was one of the top five is HPV16,HPV58,HPV53,HPV39,HPV52.2,The positive expression of P16 and Ki-67 in cervical lesions of Tibetan women at all levels of the rate of increase with cervical lesions and cervical lesions increased.Combined detection of P16 and Ki-67 is helpful for grading diagnosis of cervical lesions in Tibetan women.