Associations Among CD68~+ Cells,Interferon-gamma,and Acute Myocardial Infarction

Objective : To investigate the changes of cytokine CD68~+ cell and interferon-gamma proportion in peripheral blood mononuclear cells of patients with acute myocardial infarction and their correlation with the severity of coronary artery disease,and to explore its clinical significance.Methods :Selecting 80 patiens cardiology department of our hospital from June 2014 to August 2015.According to clinical manifestation,ecg and the results of coronary angiography,we divided them into the fllowing 3 group:(1)acute myocardial infarction(AMI)group(n=30),(2)unstable angina(UA)group(n=30),(3)a control group(n=20).The degree of coronary artery disease was assessed according to Gensini score of coronary artery.The proportion of peripheral blood mononuclear cell cytokine IFN-? and CD68~+ cells were detected by flow cytometry.The measurement data is expressed by Mean�SD.T test was used between the two groups.The mean of samples between groups was analyzed by variance analysis,the two groups were further compared by LSD test.Enumeration data were checked by chi square.Paired t test was used to compare the proportion of IFN-? in peripheral blood before and after treatment.The correlation between the proportion of IFN-? and CD68~+ cells and Gensini score in peripheral blood was analyzed by Pearson.the difference was statistically significant(P < 0.05).Results:(1)The proportion of CD68~+ cells in peripheral blood mononuclear cells of AMI(13.62�2.09)% group and UA(12.68�2.77)% group were higher than that in normal control(5.78�1.66)% group,the difference were statistically significant(P<0.01).(2)Before the standardized standardized treatment with PCI,the proportion of IFN-? in the peripheral blood mononuclear cells of AMI(19.92�3.35)% group was higher than that in the UA(17.38�4.07)% group,and the proportion of IFN-? in the peripheral blood mononuclear cells of UA group was higher than that in the control(10.22�4.54)% group,the difference was statistically significant(P<0.01).(3)After 3-5 days of standardized standardized treatment with PCI,AMI(15.0�3.56)% group and UA(11.69�2.51)% group of peripheral blood mononuclear cells in the proportion of IFN-? reduced significantly compared with pre-operative,the difference were statistically significant(P<0.01).(4)After 3-5 days of standardized standardized treatment with PCI,the proportion of IFN-? in peripheral blood mononuclear cells of AMI(15�3.56)% group and UA(11.69�2.51)% group was significantly higher than that of control group(5.78�1.66)%,the difference were statistically significant(P<0.01).(5)Correlation analysis showed that the proportion of IFN-? in the peripheral blood mononuclear cells of ACS was positively correlated with Gensini score(r =0.895,P<0.05).Moreover,the proportion of CD68~+cells in the peripheral blood mononuclear cells of ACS was positively correlated with Gensini score(r=0.355,P<0.05).Conclusion:(1)Serum level of CD68~+ cells and IFN-? were gradually increased in AMI group before the standardized hospitalization,and which were significantly higher than that of UA group and control group.It suggested that elevated levels of the both may participate in the development of AMI.(2)Correlation analysis showed that serum levels of CD68~+ cells and IFN-? in ACS before the standardized hospitalization has correlation with Gensini Score,suggesting that CD68~+ cells and IFN-? may related to the severity of coronary artery disease.(3)The level of IFN-? in AMI patients after percutaneous transluminal coronary angioplasty was significantly lower than that before.The results suggests that standardized treatment with PCI may reducethe levels of some inflammatory factors in patients.