The Effect Of Electroacupuncture On PI3K/AKT Signaling Pathway MTOR Expression In A Hypoxic-ischemic Encephalopathy Rat Model

Objective: This study explore the phosphatidylinositol 3-kinase and protein kinase B(PI3K/AKT)signaling pathways downstream of the key molecular target of rapamycin(mTOR),and the changes of cell proliferation following hypoxic-ischemic brain injury of mTOR gene itself changes.Then study of electro-acupuncture(EA)on hypoxic-ischemic encephalopathy(HIE)role and intervention mechanisms for EA in the treatment of HIE experimental scientific evidence provided in the application.Methods: The hypoxic-ischemic models were made in new-born SD rats(SPF level)aged 7 days.A total of 351,maternal feeding,which were randomly selected 36 rats in sham-surgery group,the remaining 315 rats to establish the model of hypoxic-ischemic encephalopathy by bilateral common carotid artery ligation and hypoxic in the mixed gas.And then they were randomly devided into six groups: sham-surgery group,model group,sham-electroacpuncture group,EA group,antagonists group,EA and antagonists group,each group were subdivided 1d,3d,7d,21 d time-phases.The changes of morphology and ultra-structure of neurons in the brain tissue were observed by pathology and TEM;the real-time PCR was used to determine the level of mTOR m RNA;and the expression of mTOR was tested with Western Blot.Results: Following 1d,3d,7d,21 d after hypoxic-ischemic brain damage in rats four time points,The rat of model group which pathological staining cytoplasmic concentration of nerve cells,dark,neuron injury degree under the electron microscope,fuzzy cell membrane and nuclear membrane dissolves,With the extension of hypoxic-ischemic time,the expression of mTOR genes and proteins began to increase at 1d,3d to 7d continues to increase,and was not obvious until 21 d,but the difference was statistically significant compared with sham operation group(P<0.05).The changes of nerve cell injury and ultrastructure in EA group were similar to those in model group,However,with the early intervention treatment,the expression of mTOR gene and protein increased at 1d,and the difference was statistically significant compared with the model group(P<0.05),when during the three periods at 3d,7d,21 d,The expression of mTOR increased continuously,and the differences were significant compared with the model group(P<0.05).EA and antagonists group rats nerve pathological changes and Ultra structural changes after the EA group,in 1 d,3d,7d,mTOR protein gene expression compared with the sham-surgery group,a statistically significant difference(P<0.05),21 d compared with the model group,a statistically significant difference(P<0.05).Conclusion: After hypoxic-ischemic brain damage and with the early intervention treatment of EA,it can promote the healing of damaged neurons.With the extension of treatment time,EA can contribute to brain hypoxic-ischemic mTOR gene and protein expression,which play a significant neuroprotective effect on HIE rats.