| Objective To investigate a new method of intrauterine hypoxic-ischemic encephalopathy, and evaluate the model with animal behavior researches. Methods Pregnant rats (n=15) were randomized divided into operation group (n=12) and normal group (n=3). Animal models were built by ligating both sides of the uterine arteries in full-pregnant (20 days) experimental group rats for 15 minutes. The model group and control group were the offspring of operation group and control group, respectively. Record the scores of reflex testing, rope suspension test, open-field activity, resistant to capture test, rotarod test, and Morris water maze at different time points with model group and control group rats. Results (1) Neonatal hypoxia–ischemia insult retarded the development of somatic growth and several neurological reflexes (ear twitch, eye opening, forelimb grasp, hindlimb grasp and negative geotaxis); (2) suspense time of model group shorter than control group (P <0.05) at 14 days after birth in rope suspension test; (3) at 20 days after birth, residence time of model group was significantly shorter than control group (P <0.05) at 25rpm in rotarod test; (4) times of crossing lattice and excrement number in model group significantly less than control group (P <0.05), but times of standing compared with the control group has no significant difference; resistant-to-capture test showed that rats were more difficult to capture in model group than in control group; (5) Morris water maze: time for finding hidden platform latency of each rat was gradually reduced; the mean escape latency of model group was significantly longer than control group; the swimming time in the platform quadrant in model group was shorter than control group. There were significant differences (P <0.05) in the mean escape latencies between the two groups at the training day 1, 2 and 4, but there were no significant difference at the third day of training. There were no significant differences in the probe trial test between the two groups at day 5. Conclusion: Ligation of uterine artery can establish hypoxia-ischemia animal model, and neural behavioral testing confirmed that the model is stable and reliable. Objective This study was designed to transplant the Mesenchymal Stem Cells derived from human umbilical cord into cisterna magna of the HIE rat model, and to investigate their survival,differentiation in the rat brain.and to have their Behavioral Research.Methods UCMSCs were isolated from human umbilical cord delivered after full-term normal cesarean section, and labeled by DII. Pregnant rats were randomized divided into experimental group and control group.Animal models were built by ligating both sides of the uterine arteries in full-pregnant (20 days) experimental group rats for 15 minutes. The experimental group neonatal rats were divided into stem cells group and PBS group at random. The labeled UCMSCs were injected into cisterna magna of the stem cells group rats, while PBS without UCMSCs was injected into that of PBS group. At 1 and 6 weeks after transplantation, the brain tissues were sliced and examined by immunohistochemical analysis. Record the scores of rope suspension test,rotarod test, and Morris water maze at different time points.Results 1.ethology: (1),After transplantation ,the stem cells group rats longer than PBS group rats in rope suspension test.(2),the Rota-Rod task: three weeks latter, residence time of the stem cells group rats was longer than PBS group rats.(3),Morris water maze: time for finding hidden platform latency of each rat was gradually reduced; the mean escape latency of model group was significantly longer than control group; the swimming time in the platform quadrant in model group was shorter than control group. The swimming time in the target limited have no different between the stem cells group,PBS group and control group.2.immunohistochemistry:At 1 week after transplantation, DII, nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) positive cells were found in the hippocampal dentate gyrus,motor cortex of the stem cells group rats.Conclusion The UCMSCs transplanted into cisterna magna could survive, proliferate in the brain of the HIE rat model, and Brain's capabilities can be recovery after stem cells grafted. |
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