Analysis Of The Saliva Microbiomes Community Of T2DM With DGGE

Objective:1.To compare detection rate of oral Streptococcus mutans by the two different sampling methods.2.Oral microflora in patients with periodontitis and T2 DM was studied with periodontitis and healthy controls on microbiomes community.Methods:1.Comparing the two methods to collecting salivaThe gargle and unstimulated methods were used for collecting the whole saliva with healthy individuals in two groups,calculated the detection rate of S.m(Streptococcus mutans).Statistical analyses by SPSS 22.0 statistical software,measurement data in the analysis of variance,analysis of attribute data for Chi-square test.2.Analyzing of oral microbial diversity by PCR-DGGECollecting the whole saliva from healthy individual(H group),periodontitis individual(P group)and periodontitis with Type 2 Diabetes Mellitus individual(T group)by gargle methods.The genomic DNA was extracted from three saliva groups with OMEGA DNA Micro Kit.DGGE(Denaturing Gradient Gel Electrophoresis),Quality One 1-D Analysis Software 4.52,statistical software SPSS 22.0 and Origin8.0.were used for analysis the diversity of Saliva Microbiomes.Results:1.Statistical analysis showed that the detective ratio of S.s(Streptococcus mutans)had no significant effect on the two methods.2.The index of Margalef was significant difference between H group and P group,P group and T group(P<0.05).3.Visible banding type analysis,difference microorganisms existing between individuals,but there is common bacteria in everyone.4.The UPGAMA cluster analysis of microbial community structure from the individuals showed that the similarity index of them were less than 0.80.5.PCA showed H group and T group has obvious trend of clustering.6.NMDS showed T group has the minimum.difference.Conclusion:1.The methods of passively drooling saliva and gargle can be used for collecting the sample of saliva.It's convenient for gargling method for collecting the large samples.However,there is no standard for collecting saliva at the present.2.There is difference microorganisms existing individuals,but there is common bacteria in everyone.3.There are significant differences on microbial diversity and richness,which shows that T group has a higher diversity and the lower richness than others.