Analysis Of The Effect Of Geneal Disinfectant Sterilization Porcine Skin Microbiota By Denaturing Gadient Gel Electrophoresis

BackgroundBiologic dressings have become an integral part of modern burns care. Although viable allograft is often regarded as the gold standard in temporary skin coverage. Although viable allograft is often regarded as the gold standard in temporary skin coverage,possessing many of the qualities of the ideal burns dressing the supply may be limited. Species used for xenografts Skin from a wide variety of species has been used as biologic dressings both in the past and in the present. Porcine skin became popular in the 1960s and it is currently the most widely used xenograft and consequently has the most literature devoted to it. The main advantages are that it is cheap with a readily available source (pigs have large litters and are quick to mature) and its histologic structure is supposedly close to human skin,although exactly how important this point is unclear. There are concerns, however, about the theoretical risk of zoonoses, particularly porcine endogenous retroviruses.Traditional cultivation technique is insufficient to study intestinal microbiota because it only analyse the culturable microbe. So additional techniques are requisite to appraise the regulation function to disordered microbiota introduced by antibiotic. Denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA- encoding genes was developed as rapid methodologies with high throughput that are more suitable for analysis of complex microbial communities. DGGE method has been used in skin microbiota related study.ObjectivePorcine xenografts have become an essential surgical step in the treatment of severely burned patients . Xenografts act as a biological dressing providing temporary protection to the patient by preventing loss of fluids, by controlling bacterial invasion, and by promoting the formation of granulation tissue for further grafting with human skin. The expense involved in using large quantities of animal tissue led to the establishment in this institution of an animal colony for the preparation of donor tissue which is routinely treated with antibiotic treatment and surgical application to the patient. This study was conducted to determine the efficacy of procedures used in the removal of pig skin and its decontamination with disinfection fluid and antibiotic drug.MethodsDenaturing gradient gel electrophoresis (DGGE) targeting 16S rDNA- encoding genes was developed as rapid methodologies with high throughput that are more suitable for analysis of complex microbial communities. In this study, the efficacy of procedures used in the removal of pig skin and its decontamination with disinfection fluid and antibiotic drug by DGGE.A comprehensive analysis of Porcine xenografts composition has not yet been carried out because of difficulties in determining yet-to-be-cultured micro-organisms in the samples. Sequence analysis of the bacterial 16S rRNAgene is one method that can circumvent some of these difficulties. The current study was designed to determine the Porcine xenografts microbiota by the bacterial 16S rRNA gene clone library and DNA sequencing and phylogenic analysis.Conclusions1 .The impact on the microbial ecology of pig skin by disinfections individually: The pig skin was immersed in the antibiotic and different disinfection fluid for different times . After this treatment, the efficacy of disinfectant surgical was evaluated by the tapes and diversity of the microbial ecology of pig skin. The results show there are the most significant impacts on the bacterial species and the diversity of pig skin ,after which is treatmented by effective iodine content of five percent of povidone iodine for five minutes.2.The pig skin are disinfected consecutively by different disinfection fluid . After this treatment, the results abou the sequence analysisof 16S rRNA genewa with the bacteria of pig skin show that: The majority of the clones were those of Staphylococcus,Bacillus thuringiensis,Enterococcus gallinarum strain,Clostridium sardiniense ,which constituted the predominant member in analyses of. Three species of the the bacteria of pig skin.There are still some left phylotypes,such as Swine manure pit bacterium,Escherichia coli,Acinetobacter junii.3. Two different strategies for molecular analysis of bacterial diversity, 16S rDNA cloning and denaturing gradient gel electrophoresis (DGGE), were combined into a single protocol that took advantage of the best attributes of each: the ability of cloning to package DNA sequence information and the ability of DGGE to display a community profile. They are more suitable for analysis of complex microbial communities as rapid methodologies with high throughput.