| Sargassum fusiforme is a nutrient-rich edible algae and enjoys a reputation as a "longevity food". Sargassum fusiforme polysaccharides include alginic acid, fucoidan and brown algae starch, in which fucoidan activity research is the most extensive. Recent studies have revealed that Sargassum fusiforme polysaccharide is a very promising natural antioxidant, but in the anti-aging is also a lack of research. The mechanism of anti-aging activity of polysaccharides from Sargassum fusiforme was to provide the basic theory for the research of anti-aging of seaweed polysaccharides and lay the foundation for further research on molecular mechanism.In this paper, the mechanism of anti-aging of polysaccharides from Sargassum fusiforme was analyzed from Nrf2 / ARE signal pathway and comparative proteomics. A total of 49 protein spots were measured out of which 38 up-regulated proteins and 11 down-regulated proteins. The results of mass spectrometry was detected by western blot to ensured the credibility and accuracy. A great number of these proteins was metabolic enzymes involved oxidation reduction, carbocylic acid biosynthetic, cellular amino acid biosynthetic, cofactor metabolic, generation of precursor metabolites and energy. Differential proteins involve multiple pathways of senescence,including mitochondrial redox reactions (ALDH1A1 and ALDH2), age-related diseases (ATP5B, ATP5H and Uqcrc1) and carbon monoxide metabolism(BHMT and AHCY). The focus located in the mitochondria, involved energy metabolism mitochondrial respiratory chain and mitochondrial integrity. The results showed that the polysaccharide from Sargassum fusiforme can improved mitochondrial activity and ATP enzyme level,reduced the deletion fragments of mitochondrial gene in old mice.The results showed that Sargassum fusiforrme polysaccharide could improve the survival rate and average life span significantly. Nrf2 (nuclear factor erythroid2-related factor 2) is a positive regulatory factor of the antioxidant response element and involves in the regulation of the expressions of over 500 genes, including the genes of antioxidant enzymes, and dexoxification enzymes. Among the older individuals, the overall level of Nrf2 signaling pathway with the protective effect up-regulates in the longevity individuals. Therefore, scientists believe that Nrf2 is a protector of health and longevity. Intragastric administration of SFPS enhanced the expression and the antioxidant capacity of Nrf2 system of drosophila, including the enzyme activity of SOD and CAT and the expression of GCLC, HO-1, NADPH and GSH. It can be concluded that SFPS is a good natural antioxidant, which up-regulate Nrf2/ARE signaling pathway to enhance the body's antioxidant capacity and delay aging. |
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