Effect Of Sargassum Fusiforme Polysaccharides On Proliferation And Oxidative Injury Of Vascular Endothelial Cells

Sargassum fusiforme polysaccharides(SFPS), which are distilled from sargassum fusiforme, have fine antioxidative effect in vitro and in vivo. The objectives of the present study were to examine effect of SFPS on proliferate activity of human umbilical vein endothelial cells(HUVECs) and protective and repairing effect of SFPS on HUVECs injured by H₂O₂.Proliferate activity of HUVECs was assessed by 3-(4,5-dimethylthiazo-2-yl)-diphenyl-tetrazolium bromide(MTT) assay. Lactate dehydrogenase(LDH) and nitric oxide (NO) of culture medium were assessed by UV visible spectrometry. Maleic dialdehyde(MDA) in HUVECs was assessed by thibabituric acid(TBA) assay. Superoxide dismutase(SOD) was assessed by xanthine oxidase assay.As a result, 10mg/L, 30mg/L, 100mg/L, 300mg/L SFPS promoted proliferate activity of HUVECs, and at the concentration of 100mg/L the effect was the strongest, but 1000mg/L SFPS inhibited proliferate activity of HUVECs. Adding SFPS in advance or after H₂0₂ stimulating reduced inhibitory effect of H₂0₂ on proliferate activity of HUVECs.While adding SFPS for 24 hours in advance or after H202 stimulating, LDH release of SFPS group was significantly less and NO content higher than H202 group. Accordingly, MDA content of SFPS group obviously reduced and SOD activity increased, compared with that of H202 group.In conclusion, SFPS had double effects on proliferate activity of HUVECs, SFPS exerted dose-dependant proliferate activity at lower concentration, but reduced proliferate activity of HUVECs at high concentration. And at lower concentration SFPS protected and repaired proliferate activity of HUVECs injured by H202. Furthermore, SFPS had significant protective and repairing effect on HUVECs injured by H2O2, which is possibly related to antioxidative effect of SFPS.