Influence Of Metastin On The Capacity Of Metastasis And Invasion In Highly Metastatic Nasopharyngeal Carcinoma SUNE-1-5-8F Cells

Objective: To investigate and discuss the effect of metastin on inhibiting the capacity of metastasis and invasion in highly metastatic nasopharyngeal carcinoma SUNE-1-5-8F cells.Methods:(1)The SUNE-1-5-8F cells' were treated with different concentration of metastin(102nM,103 nM,104nM).Contrast the cells before and after treatment.Finally test the capacity of metastasis and invasion by transwell assay.(2)Forty female mouse were randomly dividend into metastin group and control group.Both were injected with 2×106/ml SUNE-1-5-8F cells under the armpits.Mouse in metastin group were injected with 1.17mg/kg(metastin/weight)metastin at day 2.The tumor inhibition rate,metastasis inhibition rate and life extension rate were detected by BALB/c-nu nude mouse model assay.The expression of kisspeptin protein was analyzed by immunohistochemistry.Results:(1)Metastin inhibited the SUNE-1-5-8F cells' capacity of metastasis and invasion in cell assay(P<0.05),whose capacity had a positive correlation with the concentration of metastin.(2)Metastin didn't inhibit the proliferation of tumor in BALB/c-nu nude mouse assay(P>0.05),but it reduced the pulmonary metastasis(P<0.05).And metastin influenced the life extension rate of nude mouse(P<0.05).The result of immunohistochemistry showed that the expression of kisspeptin protein of the metastin group was significantly different from the control group(P<0.05).Conclusion:(1)Metastin inhibited the SUNE-1-5-8F cells' capacity of metastasis and invasion in cell assay,whose capacity had a positive correlation with the concentration of metastin.(2)Metastin reduced the pulmonary metastasis and prolonged life,but it didn't inhibit the proliferation of tumor in BALB/c-nu nude mouse assay.