Expression And Significance Of KiSS-1 Gene And MMP-9 In Epithelial Ovarian Cancer

Objective: To study the expression of metastasis suppressor gene KiSS-1 and its product metastin in epithelial ovarian cancer, and the correlation between the metastin expression and MMP-9.Methods: Expression of KiSS-1 gene was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) in 27 cases of epithelial ovarian cancer, 5 cases of borderline cancer, 10 cases of epithelial benign tumors and 11 cases of normal tissues. Expression of metastin and MMP-9 was detected by immunohistochemistry method in 54 cases of epithelial ovarian cancer, 11 cases of metastatic focus, 10 cases of borderline cancer, 12 cases of epithelial benign tumors and 12 cases of normal tissues, and Expression of metastin was compared with clinicopathological findings and expression of MMP-9.Results: Expression of KiSS-1 gene and metastin was detected in a significantly higher in epithelial ovarian cancer and borderline cancer than epithelial benign tumors and normal tissues (P<0.05) . The positive rate of metastin in metastatic focus is a little lower than primary tissue (P>0.05) . There is no relationship between metastin expression and patients' age, FIGO stage, histologic grade, pathological type, lymphatic metastasis and expression of MMP-9.Conclusion: Expression of KiSS-1 gene in epithelial ovarian cancer is up-regulated, and is not associated with the expression of MMP-9.