Butorphanol Impact On Sepsis In The Rat Myocardial Cells

Objective: To observe the changes of cardiac markers in the selective kappa opioid receptor agonist Butorphanol,further explore brown bhutto's influence on myocardial cells in sepsis and its possible mechanism for clinical to find a feasible way to protect myocardial injury in sepsis.Method: Select male SD rats?provided by nanchang university experimental animal sciences?,81,12 to 15 weeks of age,were randomly divided into three groups.A randomized,positive control experiment design method,This clinical study of experimental group and the control group,according to the proportion of allocation and the statistics of the minimum requirements,effective cases in each group 27 cases,control group?A?,sepsis group?B?,and bhutto brown + sepsis group?C?,A total of 81 cases.All rats separation on the left side of the femoral vein in 22 G trocar,control group rats slow intravenous saline injection,5ml/kg.The rest of the rat femoral vein slowly push note bhutto brown.50ug/kg,in addition to the control group,all other rats using cecum ligation perforation of of sepsis model.Preoperative eating for12 hours,free water,intraperitoneal injection of 2% sodium pentobarbital 50 mg/kg of anesthesia,Supine position fixed,abdominal skin disinfection,along the midline abdominal longitudinal length is about 1.5 cm incision,separation of thin film and appendix,gently squeeze the ascending colon,the intestinal contents full of cecum,take3.0 silk thread in the back of blind ring ligation under appendix root.Then,use 20 g needle through ligation 2 times,since the puncture hole extrusion of intestinal contents into the abdominal cavity,place a article about 2 cm long rubber drainage,prevent pinhole closure,then insufflate Hai Na celiac,layered suture abdominal incision,iodine disinfection incision.Group A only exposed the cecum,choose 24 h time observation points,model replication 24 h after abdominal aortic blood3 m L,after centrifugal supernatant on a 70 ? saved for later use,the determination of serum CK-MB and c Tn I.By using enzyme-linked immunosorbent?EI ISA??bought kits from abbott laboratories,USA?.The rest of the myocardial tissue homogenate supernatant after,ET-1 was determined by ria method research domain?kit purchased from Shanghai chemical reagent co.,LTD.?.Using ELISA method to detect TN-a?kit provided by Shanghai xin ran biological technology co,LTD.?.Result:Sepsis group elevated myocardial enzymes degree is high in the control group?p < 0.05?.Butorphanol group of rats myocardial enzyme spectrum higher degree was lower than those of sepsis group?P < 0.05?.Compared with sepsis group,Butorphanol,plasma c Tn I,TNF alpha significantly decreased?P < 0.05 or 0.01?.Histological observation shows Butorphanol group can obviously improve sepsis rats myocardial congestion,edema and inflammatory cells infiltration and other pathological changes.Conclusion:Butorphanol can protect myocardial cells in the sepsis rat,The possible reason is Butorphanol by activating kappa receptors predominate on rat myocardial cell,reduce the Ca²⁺ overload and The KATP channelin the myocardial cells,Improve the ability of myocardial cell oxidation,relieve myocardial cell edema,and inflammation,to protect myocardial cells.