Exploring The Effect Of PI3K/Akt Signal Pathway To Phantom Limb Pain

Objective: this study was to investigate whether PI3K/Akt signal pathway involved in the pathological process of phantom limb pain,research mechanism of phantom limb pain,provide ideas for the follow-up treatmentMethods: Adult male Sprague-Dawley(SD)rats,weight between 150 g and 200 g were selected.Adaptability raised after 3 days,and randomly selected 48 SD rats,modeling phantom limb pain,abrosia 8 hours pre-operation,drinking water freely,then 1%sodium pentobarbital(30 to 45 mg/kg)was injected in the abdominal cavity,skin preparation,sterilized with povidone iodine,find the right sciatic nerve of the femur after separation section,resect the sciatic nerve about 0.5 cm at a distance of about 0.5cm from infrapiriformis foramen,the end of sciatic nerve was sutured in the muscles,and then skin,muscle and fascia were sutured by layer.after modeling,all SD rats awaked were raised in the same conditions.With batch of 24 SD rats were selected to model a control group that false phantom limb pain model group,only separated from the right sciatic nerve of the posterius part of the femur,then suture muscle,fascia and skin by layer.Postoperative awaken in clean cage,all rats raised in the same condition.In the phantom limb pain group,evaluate the model is successful or not of SD rats through there autophagy behavior,one day later,SD rats with the transection of the right sciatic nerve,will gradually had different degree of autophagy behavior,according to references about autophagy criteria to judge whether phantom limb pain appered in the group above.Modeling success time is about 2 weeks.The successful model of phantom limb pain were selected(weigh between 200 g ~250 g),then randomly divided into 2 groups(24 / group),PI3 K inhibitor Wortmannin fluxing agent of DMSO(amount equal to the group B)was injected through tail vein every Monday in group A(phantom limb pain control),group B(PI3K inhibitors group)and group A were injected of PI3 K inhibitor Wortmannintail through tail vein at the same time,select false phantom limb pain model group of 24 SD rats as group C(control group): inject the equal amount of saline solution of group B at the same time of group A.After injecting through tail wein 4 weekend(Ta),6 weekend(Tb)and 8 weekend(Tc)Respectively,then 8 rats were selected randomly in each group,detect the level of p-Akt in L3 ~ L5 segment of spinal cord and spinal cord of L4 ~ L5 dorsal root ganglion(all 8 rats were detected by immunohistochemical method,4 rats were selected randomly,they were detected by Westernblot method).After taken out the specimens,put the experimental rats to death.Results: Compared with group C,p-Akt protein expression were significantly increased in other groups(P < 0.05);Compared with group A,P-Akt protein expression were decreased obviously in group B(P < 0.05).Conclusion: PI3K/Akt signaling pathways involved in the pathological process of the formation of phantom limb pain in rats.