An Experiment Of Edaravone Pretreatment On Acute Myocardial Injury Induced By THP In Rats

Objective: To observe the effect of edaravone(EDA)on acute myocardial injury induced by pirarubicin(THP)in rats,and to investigate whether EDA preconditioning had protective effect on acute myocardial injury in rats.Methods: A total of 40 female SD rats of 230-260 grams,were divided into four groups randomly,and 10 rats in each group as follows: Group normal control(group GNS),group perarubicin(group THP),group EDA-5mg(group EDA-1),group EDA-10mg(group EDA-2).The rats of group GNS were injected with normal saline on 1,2,3,4,5,6,7 days.5% glucose were injected intraperitoneally in two hours later with the last injection;The rats of group THP were injected with EDA equivalent saline on the 1,2,3,4,5,6,7 days.THP dose of 15mg/kg were injected intraperitoneally in two hours later the last injection;The rats of group EDA-1,group EDA-2 were injected with EDA dose of 5mg/kg,10mg/kg on the 1,2,3,4,5,6,7 days respectively.On the tenth day of the experiments,the rats were anesthetized and the blood samples were taken from abdominal aorta,and to detect the levels of serum NT-pro BNP and cTnI.The carotid artery specimens were made and the pathological changes were observed under light microscope.Pathological changes of myocardial tissue of anterior wall of ventricle were observed and scored by light microscope.The remaining myocardial tissue was made into homogenate,the activities of OH,GSH-Px,SOD and the level of MDA were measured.Results:(1)Third days after the treatment of THP,the weight of rats in group THP were significantly lower than that in other three groups(P<0.05).There were no significant difference in weight between group GNS,group EDA-1 and group EDA-2(P>0.05).(2)The HWI of group EDA-1,group EDA-2 and group THP were higher thanthat in group GNS(P< 0.05),and that in group THP of rats were higher than that in rats of group EDA-1 and group EDA-2(P<0.05),there were no significant difference between group EDA-1 and group EDA-2(P> 0.05).(3)The level of serum cTnI in group THP were higher than that in rats of other three groups(P <0.05),and that in rats of group EDA-1 were higher than that in group EDA-2(P <0.05).The level of serum NT-proBNP in rats of group THP were higher than that in other three groups(P<0.05).There were no significant difference between group GNS,group EDA-1 and group EDA-2.(4)The activities of ·OH in rats of group THP,group EDA-1 and group EDA-2 were significantly higher than that in rats of group GNS(P <0.05),and that in rats of group THP were higher than that of group EDA-1 and group EDA-2(P <0.05),and that in rats in group EDA-1 were higher than that of group EDA-2(P <0.05).(5)The activities of GSH-Px in rats of group THP were significantly lower than that in other three groups(P< 0.05),and that in rats of group THP,group EDA-1 were lower than that in group GNS(P<0.05),but there were no statistical difference between group EDA-2 and group GNS(P>0.05).(6)The activities of SOD in rats of group THP were lower than that in other three groups(P<0.05),and that in group EDA-1,group EDA-2 and group GNS had no significant difference(P>0.05).The level of MDA in rats of group THP were lower than that in other three groups(P <0.05),and the level of MDA in rats of group EDA-1 were higher than that in group EDA-2(P<0.05).(7)Under light microscope,there were obvious changes of right carotid artery and myocardium in rats of group THP,and the pathological changes were lighter in group EDA-1 and group EDA-2.(8)Myocardial pathological score: that in group THP were significantly higher than that in other three groups(P <0.05),and that in group EDA-1 and group EDA-2 reduced the myocardial pathological score(P< 0.05),but there were no significant difference between group EDA-1 and group EDA-2(P >0.05).Conclusion:(1)THP can induce myocardial injury and carotid artery injury in rats.(2)Pretreatment with EDA 5mg/kg and 10mg/kg had protective effects on acute myocardial injury induced by THP in rats.The protective effect was better in pretreatment of 10mg/kg.