Preparation Of Apocynum Leaf Total Flavonoid Nanoemulsion And Study Its Protective Effects Of Myocardial Injury Induced By Pirarubicin In Rats

Cell growth-inhibiting antibiotic anthracyclines(daunorubicin,doxorubicin,pirarubicin,etc.)play an important role in clinical anti-cancer drugs,but also have a significant side effect-cardiotoxicity,The use of these drugs to treat cancer patients is extremely troublesome.Therefore,the prevention and treatment of early stage cardiovascular adverse events becomes more and more important.Apocynum leaf flavonoids have many pharmacological activities such as anti-inflammatory,antioxidation,scavenging free radicals,anti-depression,neuroprotection,etc.They are widely used in the treatment of cardiovascular diseases.Some researches have confirmed that the total flavonoids induced by doxorubicin in rat myocardial Damage has a therapeutic effect.However,Apocynum leaf flavonoids are flavonoids with low drug solubility and are insoluble drugs.In the actual application process,it is difficult to accurately grasp the total flavonoid dose of Apocynum leaf,and the drug is not easily absorbed by the body,and the bioavailability is low.In order to improve the solubility of total flavonoids in A.venetum leaves,the nanoemulsion formulation suitable for encapsulating total flavonoids in A.venetum leaves was selected to prepare ANP-based total flavonoid nanoemulsion.Using the phase inversion method and combining the pseudo-ternary phase diagram,the formulation and dosage of total flavonoid nanoemulsion from A.venetum leaves were: emulsifier(36.4%),oil phase(18.2%),and aqueous phase(45.4%).The emulsifier is a 1:1 mixture of surfactant(Tween 80 and Span 80 mixture)and co-surfactant(PEG 400).The oil phase is IPM and the aqueous phase is triple distilled water.The resulting Apocynum leaf flavonoid nanoemulsion was evaluated for its quality: particle size distribution,polydispersity,Zeta potential,p H measurement with a p H meter,high-speed centrifugation,refrigerating cycle,and long-term storage stability as measured by a Malvern Particle Size Detector.HPLC determination of drug content and entrapment efficiency.The results are as follows: The average particle size of the blank nanoemulsion was 24.73±0.34 nm.The average particle size of the total flavonoid nanoemulsion was 31.91±0.53 nm.The average particle size was uniformly dispersed with good stability.The total flavonoids of A.venetum in the nanoemulsion were as follows: The drug content was 9.355 mg/m L and the encapsulation efficiency was 93.55%.The model of rat myocardial injury induced by THP was replicated,and the protective effects of total flavonoids of A.venetum L.and total flavonoids of A.venetum L.on myocardial damage induced by THP in rats were investigated.Results: Both AP.LF and AP.NE can significantly increase rat heart rate,QRS complex voltage,significantly reduce LVEDP in rats,increase LVSP,±dp/dtmax,improve heart function in rats,and significantly increase SOD activity.,significantly reduce the content of MDA,BNP,CK-MB,c Tn-T,LDH,regulate myocardial enzyme metabolism;reduce the degree of myocardial fiber breakage,intracellular vacuolization,and nuclear overflow.In addition,at the same AP.LF and AP.NE doses,AP.NE significantly increased heart rate,QRS complex voltage,significantly shortened QT interval,and significantly reduced LVEDP,±dp/dtmax,and BNP in acute myocardial injury rats.,CK-MB,and other indicators were not significantly different;chronic myocardial injury in rats,AP.NE can significantly increase LVSP,significantly reduce BNP,c Tn-T,LDH levels,therefore,AP.LF and AP.NE on THP caused by large Myocardial injury in mice has a protective effect,and the same dose of AP.NE can more effectively improve myocardial heart function and resist changes in myocardial enzymes.The experiment successfully screened the Apocynum leaf flavonoid nanoemulsion with certain stability and can effectively improve the solubility of total flavonoids in A.venetum leaves,and it was used for the protection of rats with myocardial damage induced by THP.The results confirmed that the total flavonoids of A.venetum L.and total flavonoids of A.venetum L.had a protective effect on THP-induced myocardial injury in rats,and the same concentration of A.flavone leaf total flavonoid nanoemulsion compared to A.louv The protective effect of myocardial injury in rats was more significant,and the effectiveness of the total flavonoid nanoemulsion of A.venetum L.and the total flavonoids of apocynum venetum on THP-induced myocardial injury in rats was improved.