Effects Of Different Order Of Electroacupuncture At Zusanli,Zhongwan Regulate Gastric Motility In Mice And Its Mechanism Of Autonomic Nerve

Objective:Acupuncture treatment of gastrointestinal disease has been recognized for its efficacy ingastrointestinal functions,and "Zusanli" and "Zhongwan" are the commonly used acupoints for such treatment.During the ancient "Neijing" period,"acupoint needling order" was an important component of the acupuncture prescription,but in today's clinical and experimental study of acupuncture,the "acupoint needling order" is neglected and is far less emphasized than the study of "stimulus intensity" and "acupoint combinations".Autonomic nerve plays an important role in acupuncture regulation of gastrointestinal motility.The C57BL/6WT mice,M2&3 receptor knocked-out mice,and ?1&2 receptor knocked-out mice,were the subjects in the experimental process and were used to explore the effects of regulating gastric motility under different electroacupuncture orders,and to review the potential intrinsic mechanism of autonomic nerve..Methods:105 mice were randomly divided into 3 groups in the experimental process,namely the C57BL/6WT mice,M2&3 receptor knocked-out mice,and ?1&2 receptor knocked-out mice,each group consists of 35,male mice.The self-made water sac pressure probe was placed in the gastric antrum of the mouse,and the gastric motility was converted into a waveform by a pressure transducer.When the gastric motilityof the mouse was stable,and electroacupuncture was started at a single point,Zusanliand Zhongwan separately,and then electroacupuncture both acupoints concurrently under different needling order.Results:1.For normal wild type mice,electroacupuncture singly on Zusanli promoted gastric motility,however electroacupuncture singly on Zhongwan inhibited gastric motility.Different electroacupuncture order of Zusanli,Zhongwanshowed inhibitory effects on gastric motility largely due to Zhongwan(P<0.05).Electroacupuncture both acupoints Zusanli and Zhongwan concurrently with different orders,there was no significant difference of their effects on gastric motility in normal wild-type mice.2.For M2&3 receptor knocked-out mice,electroacupuncture at Zusanliin hibited gastric motility;electroacupuncture at Zhongwan also inhibited gastric motility,but the inhibitory effects was delayed(P<0.05 or P<0.01).Different orders of electroacupuncture on both acupoints significantly inhibited gastric motility,except for the group of needling Zusanli first,followed by needling Zhongwan later.The different electroacupuncture order produce ddifferent effects of electroacupuncture(P<0.05).3.For ?1&2 receptor knocked-out mice,electroacupuncture on Zusanli promoted gastric motility(P<0.05),but the effect was delayed too;effectsof electroacupuncture on Zhongwan was not significant.Different orders of electroacupuncture on Zusanli,Zhongwan.affected the effects of electroacupuncture and appeared to either inhibit or promote gastric motility(P<0.05).Conclutions:1 Zusanli's effect of promoting gastric motility was closely related to the parasympathetic nerves,while the inhibition of gastric motility of Zhongwan was closely related to sympathetic nerves.2.Under normal conditions,the promoter effects of Zusanli could be neutralized by the inhibitor effect of Zhongwan,and Zusanli could weaken the inhibitory effects of Zhongwan to certain extents.3.Different orders of electroacupuncture at Zusanli,Zhongwan had different impacts on the effect of gastric motility in M2&3 receptor knocked-out group and P1&2 receptor knocked-out group.Different orders of needling could affect the effects of acupuncture.