Separation And Purification Of Polysaccharides And Flavonoids From Actinidia Arguta And Its Effect Of Runchang Catharsis

Actinidia arguta is an important wild fruit in the northeastern region,known as the world treasures in the name.At present,the global outbreak of chronic diseases,people began to pay attention to diet health care.Actinidia arguta as the third generation of food and health care products with the development of great significance.The preliminary study of the laboratory shows that the Actinidia arguta polysaccharides have important effect on the spleen,hypoglycemic.Flavonoids have important on the liver and the role of liver protection.Based on the pathogenicity of chronic diseases and the pathogenesis of constipation,four strains were screened by studying the traits,sub-biomass and nutrient contents of 19 species of Actinidia arguta,and the polysaccharides and flavonoids extraction and purification of the mice.The mice were treated with the extract to explore the impact of constipation mice in order to provide a theoretical basis for the development of laxative functional food.The main results of this study are as follows:(1)The results showed that the Actinidia arguta fruit of 19 species had phenolic,volatile fats and oils,polysaccharides and their glycosides,flavonoids and their glycosides,excluding terpenoids,tannins and saponins,among which 1 No.2,No.4,No.7,No.8,No.13,No.19 strains were higher than the orthers.By analyzing the nutrient content,the average Vc content of the 19 strains was 266.97 mg·100g-1,the average reducing sugar content was 27.07%,the average titratable acid content was 0.23%,the average soluble solid content was 19.58%,the average polysaccharide content was 43.71%,the average flavonoid content of 2.56 mg/mL,NO.2,NO.4,NO.7and NO.19,the content of the material were higher than the average.Finally,combined with the color,shape and other traits analysis to determine the above four strains for the late test with Actinidia arguta fruit,and re-numbered No.1,No.2,No.3,No.4.(2)The yield of polysaccharides from No.1,No.2,No.3 and No.4 was43.14%?45.01%?42.79%and 58.02%respectively by hot water extraction.The polysaccharides were separated and purified by DEAE-52 cellulose column chromatography.No.1 polysaccharide to get three sub-components;No.2 polysaccharide to get two sub-components;No.3 polysaccharide to get three sub-components;No.4 polysaccharide to get two sub-components.There were no 260nm and 280nm protein and nucleic acid in the subscale of the subspecies.The visible light was also free from the pigment.From the Sephadex G-100 gel column elution curve,it was found that each component had only one main peak,indicating that the above groups Min is a single component of polysaccharide sub-components.It can be seen from the infrared spectroscopy analysis that No.1,No.2,No.3 and No.4 have the characteristic absorption peak of carbohydrate,which is composed of a variety of monosaccharides by a-glycosidic bond and ?-glycosidic linkage.(3)The extraction rates of Flavonoids No.1,No.2,No.3 and No.4 were 2.79%?3.01%?3.27%and 2.62%respectively by ethanol extraction.Through the electrospray ionization test,preliminary determination,No.1 flavonoids contain rutin,quercetin-glucoside/galactoside and soy isoflavone glucoside pentaacetate,hyperoside octaacetate;No.2 flavonoids contain rutin,quercetin-glucoside/galactoside and propranolol octyl ether;No.3 flavonoids contain rutin,quercetin-glucoside/galactoside and pueraria flavonoid hexaacetate;No.4 flavonoids contain rutin,quercetin-glucoside/galactoside and probucilidomethylene ether,octamethoxy flavonoids.(4)Mice with gavage experiments showed that,AAP,AAF,AAP&F first row of black time,6 hours of defecation grains,fecal weight,fecal water content were better than the model control group.The results showed that AAP and AAF had the effect of promoting intestinal peristalsis,shortening the first defecation time,increasing the number of defecation in 6 hours and softening the feces.AAP more than AAF,AAP&F defecation time faster,between varieties:No.1>4>3>2;AAP&F than AAP,AAF6 hours more than the number of defecation,between varieties:4>3>2>1;The AAP,AAF and AAP&F fecal weight and water content were not significantly different.Among them,AAP of NO.4 the best effect,the AAP&F second,AAF group 1 and 3,the effect is better.Mice in the test eating,drinking water were on the rise,It can be seen that AAP and AAF can relieve symptoms of oligodynia and loss of appetite caused by constipation in mice.