Expression And Significance Of GPR30 And EGFR In Uterine Junctional Zone Of Adenomyosis

With the improvement of people's quality of life,it is not only the tumor suffering people's physical and mental health but also infertility and dysmenorrheathe by the adenomyosis,which affect the quality of women' life.According to reports in the literature,the pathological results after hysterectomy by data statistics show that the incidence of adenomyosis ranging from 8% to 27%,which is more common in women aged between 30 to 50 years old.Although the level of diagnosis and treatment of adenomyosis has been increasing,its morbidity has been increasing and the age of onset has been younger and younger.The etiology of adenomyosis is complex,involving pregnancy,production,and it may be related with hormonal disorders,endometrial basal invagination,lymphatic and venous spread,immune factors and genetic changes.While the mechanism has not been yet reached a consensus.With the development of imaging technology,magnetic resonance imaging has seen a signal of low intensity and a thickness of more than 12 millimeters,which can be used for clinical diagnosis of adenomyosis,and the accuracy rate is 85%.It is suggested that adenomyosis is caused by the damage of the structure and function of the uterine junctional zone.Therefore,it is a hot issue in the world to find out the regulatory factor in the uterine junctional zone which is connected with the development of adenomyosis and we may try to restore the structure of the uterine junctional zone to inhibit the adenomyosis by biological effects.G protein-coupled receptor 30(GPR30),as a member of the 7 transmembrane G protein coupled receptor superfamily,GPR30 is involved in the rapid nongenomic effect,by transmiting chemical cues or nerve impulse,thus affecting the downstream effector molecules to active many signal transduction pathways and exert their biological effects,such as cell cycle progression or cell proliferation in the corresponding target tissue.When estrogen integrates with GPR30,cascade reaction is induced by the EGFR/MAPK/ERK,to promote cell proliferation and differentiation;or by the pathway of PI3K/Akt to promote cell proliferation;or by regulating the intracellular second messengers such as cAMP and Ca2+,to transfer extracellular signal to intracellular cells.GPR30 is not only involved in regulating the normal physiological function of human body,but also closely related to the occurrence of the hormone-dependent diseases.Epidermal growth factor receptor(EGFR),as one of the receptor tyrosine kinase family members,can be combined with a variety of ligands,resulting in intracellular tyrosine kinase activation,thereby activating multiple signal transduction,transfering extracellular signals into intracellular signals and affecting cell proliferation,differentiation and apoptosisin many important biological processes.GPR30 mediates the PI3K/Akt pathway in endometrial carcinoma cells under the trans activation of EGFR.As the intersection of many different signaling pathways,EGFR plays an important role in the pathogenosis and etiotogy of many estrogen-dependent tumors.This study attempts to explore the effect of GPR30 and EGFR in the uterine junctional zone of adenomyosis by detecting their expression in the tissue,and provide the research foundation for GPR30/EGFR signal pathway in adenomyosis.ObjectiveThis experiment detects the expression of GPR30 and EGFR in JZ of patients with adenomyosis in the endometrial proliferative and secretory phase by immunohistochemistry and real-time fluorescence quantitative PCR method.And we analyze the differences between adenomyosis and normal JZ tissues and research the effect in the occurrence and progress of adenomyosis,in order to providing the basis for the study on the relationship between the GPR30 signal transduction pathway and JZ tissues of adenomyosis.Methods84 cases underwent hysterectomy or subtotal hysterectomy patients,are involved in 50 cases of adenomyosis patients(adenomyosis group,28 cases of specimens from JZ in the endometrial proliferative phase and 22 cases from JZ in the endometrial secretory phase),and 34 cases of patients with cervical lesions(control group,18 cases of specimens from JZ in the endometrial proliferative phase and 16 cases from JZ in the endometrial secretory phase).JZ tissues are located and obtained in both groups by MRI.GPR30 and EGFR protein are detected by immunohistochemical method in JZ of adenomyosis group and the control one.The expressions of GPR30 and EGFR mRNA in JZ of both groups are detected by real-time fluorescent quantitative PCR.Statistical methodsUsing SPSS22.0 statistical software for analysis.Quantitative datas are presented in the form of x �s,and different groups are compared by t test or corrected t test;Qualitative datas are analyzed by Chi-Square Tests or corrected Chi-Square Tests.Chi-Square Tests are used to analyze the relationship between GPR30 and EGFR in uterine junctional zone of adenomyosis.Inspection standard ?= 0.05.Results1.GPR30 and EGFR are both expressed in the cell membrane and cytoplasm.The expressions of GPR30 and EGFR in the JZ of adenomyosis group are observably higher than that in the control group,which the differences are statistically significant(P<0.05).2.In the control group,the positive rates of GPR30 and EGFR in JZ have periodic variational regularities,that in the endometrial proliferative phase are higher than the endometrial secretory phase,and the differences are statistically significant(P<0.05);however,in JZ of the adenomyosis group the positive rates of GPR30 and EGFR are non-periodic variational,which is no significant difference in endometrial proliferative phase compared with that in endometrial secretory phase(P>0.05).3.The relative expressions of GPR30 mRNA and EGFR mRNA in JZ of adenomyosis are higher than that in the control group,which the differences are both statistically significant(P<0.05).4.In the control group,the expressions of GPR30 mRNA and EGFR mRNA in JZ have periodic variational regularities,that in the endometrial proliferative phase are higher than the endometrial secretory phase,which the differences are statistically significant(P<0.05);in the adenomyosis group,the expressions of GPR30 mRNA and EGFR mRNA in JZ are non-periodic variational,which is no significant difference in endometrial proliferative phase compared with that in endometrial secretory phase(P>0.05).5.The expressions of GPR30 and EGFR in JZ of adenomyosis are positively correlated.Conclusions1.In the normal uterine junctional zone,the expressions of GPR30 and EGFR both have periodic variational regularities,which the changes may be regulated by ovarian hormone.2.In the uterine junctional zone of adenomyosis,the high expressions of GPR30 and EGFR are non-periodic variational,which may be related to the pathogenesis of adenomyosis.3.In adenomyosis,GPR30 and EGFR may be related to the occurrence and progress together.