| Objective:Through the observation of postoperative colorectal cancer patients with Qi deficiency syndrome distribution characteristics,analysis of the relationship between syndrome factors and clinical features of patients with miR-133a,miR-152 and prognosis,to explore the pathological mechanism of traditional Chinese medicine and Western medicine in patients with colorectal cancer after surgery,curative effect evaluation system for the development of combined Chinese with Western medicine treatment scheme and construction of Chinese medicine characteristics provide methodological basis and the theoretical basis,also lay the foundation for the biological and genetic mechanisms of TCM pathological changes for the further study of colorectal cancer.Methods:To collect 140 cases of patients with colorectal cancer after radical resection standardized,There were 101 postoperative colorectal patients with qi deficiency were identified using the method of differentiation of syndrome elements.Data analysis showed that the distribution of syndrome elements in CRC patients with Qi deficiency syndrome after the operation.The plasma miR-133a and miR-152 expression levels were detected by TaqMan method.Results:1.Cases distribution:101 cases patients with Qi deficiency syndrome patients,the average age was 54.98± 10.57 years,male 55 cases,female 46 cases.22 cases had no chemotherapy,chemotherapy in 79 cases;51 cases of colon cancer and rectal cancer in 50 cases.36 cases of stage ?,? in 65 cases.2.The patients with Qi deficiency syndrome have both the syndrome,of which 3 kinds is the most,the highest frequency of the three elements are:Yang,yin and Qi stagnation.Cluster analysis showed that the Qi deficiency group can be divided into two categories:Yin deficiency,Yang deficiency,blood deficiency and other.3.The frequency of female blood deficiency was higher than that of male,the frequency of ? was higher than that of ?,the difference was significant the frequency of colorectal cancer was higher than that of rectal cancer(P<0.05).The frequency of phlegm,blood stasis and lung syndrome in recurrent and metastatic group was higher than that in syndrome non group,but there was no significant difference(P>0.05).4.The relative expression level of plasma miR-133a of non liver syndrome group was 2.22 times higher than that in the liver syndrome group,and the non blood stasis syndrome group was more than that of the blood stasis syndrome group,which was significantly different(P<0.05).There was no significant difference in the expression of miR-152 in each syndrome group(P>0.05).The expression level of miR-133a in plasma was 2.72 times of recurrence and metastasis group,and the difference was significant(P<0.05).There was no significant difference between the other groups(P>0.05).5.Regression analysis showed that CRC patients with Qi deficiency syndrome and wet,liver and TNM staging were the influencing factors of recurrence after CRC,and the difference was significant(P<0.05).Conclusion:1.Qi deficiency syndrome patients with the highest frequency of the three kinds of syndromes:Yang deficiency,yin deficiency and qi stagnation,can be divided into yin and Yang,deficiency of blood and other two categories,that qi deficiency syndrome of TCM in patients with complex pathological changes and with Qi deficiency.Gender,tumor location and TNM stage were the factors affecting the distribution of blood deficiency,large intestine and liver syndrome.2.The relative expression of plasma miR-133a in liver,blood stasis syndrome group and recurrence and metastasis group were down regulated,indicating that the down-regulation of plasma miR-133a expression may regulate the distribution of liver and blood stasis syndrome and prognosis in patients with Qi deficiency syndrome after the operation.3.The factors influencing the recurrence and metastasis of the patients with Qi deficiency syndrome of colorectal cancer were dampness,liver syndrome and TNM staging. |
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