Analysis About Differential Expression Of MiRNAs In Colorectal Cancer With Or Without High Expression Of Gastrin

Objective: To clear the detailed of molecular basis of gasrtrin-regulated growth of colorectal cancer from “GAS-mi RNA-target gene” signaling pathway and than analyzed its clinical significance.Methods: Tissue blocks from 43 patients icluding 16 cases of colon cancer, rectal cancer in 27 cases(24males and 19 females, aged from 31 to 73,with a mean age of 53.1±17.8) who underwent surgical resection in the Wannan Medical College in the period of April 2011-July 2011.The patients’ pathologic features(such as sex, age, tumor location, and Dukes’ stage, histological type, status, invasion depth, tumor differentiation, and distant metastasis etc.) were obtained on their informed consent. Gastrin was than determined by using enzyme linked immunosorbent assay(ELISA) and than human colorectal cancers with high and no-gastrin expression were analyzed by using the microarray.Results: 1.The colorectal cancer tissue was determined by using ELISA.The dose of GAS which >50.00pg/g in 9 cases probaly accounting for 21% of the total. The concentration was greater than 200.00pg/g in 2 cases, were 226.10, 202.20.pg/g; 50 to 200.00pg/g in 7 cases, respectively 178.85, 156.10, 122.85, 118.70, 93.95, 76.55, 51.65pg/g; the dose of GAS of rest samples was not found. 2、Human colorectal cancers with high-and-no gastrin expression were analyed by uising Axon Gene Pix 4000 B microarray scanner.4.Scanned images were then imported into Gene Pix Pro 6.0 software(Axon) for grid alignment and data extraction. Replicated mi RNAs were averaged and mi RNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed mi RNAs were identified through fold change filtering. Selected 3 up-regulated and 3 down-regulated expressed mi RNAs verified by real-time PCR.3、There were significant differences in gene expression between human colorectal cancer with high-and no-expression gastrin. As compared to colorectal cancers without gastrin expression, 159 differentially expressed genes were up-regulated and the remaining 77 were down-regulated. Between positive expression of gastrin of cancer tissues and adjacent normal mucosa, genechip research result showed differential gene increased more than 3 times of 349, 3 times of the down regulated genes were 108. Between negative expression of gastrin of cancer tissues and adjacent normal mucosa, increased more than 3 times of 212, 3 times of the down regulated genes were 61. Between positive and negative expression of gastrin of cancer tissues and adjacent normal mucosa, increased more than 3 times of 22, down regulated genes were 12. The difference was statistically significant(data comparison between the groups P <0.05).Conclusion: 1、There are differential genes expression between the positive and negative expression of gastrin in colorectal carcinoma. 2、Different mi RNAs including the unreported which expressed notably between human colorectal cancers with high-and no-gastrin expression may provide more important clues for finding new genes related to gastrin-based colorectal cancers.