Research On Mechanism Of The Effect Of Charred Semen Arecae On Gastrointestinal Motor Function

Objectives:To investigate the effect of Semen Arecae (SA) and Charred Semen Arecae(CSA) on gastrointestinal motility,brain-gut peptide in chronically stressed and normal rats, and the effect on the rats gastrointestinal smooth muscle in vitro. In order to explore the possible mechanism of CSA can be "more effective on gastrointestinal motor function" and provide reference for clinical application of CSA and research on the Chaojiao mechanism of Traditional Chinese Medicine.Methods:1 GroupNinety rat were randomly divided into control group,normal group and stress group. Ten in control group(C),thirty in normal group, and fifty in stress group. After the rats in stress group exposed to chronic stressors for 21 days, ten rats were randomly selected as a pathology group and another forty rats were randomly divided into model group, SA group, CSA group and Mosapride group. Thirty rats in normal group were randomly divided into SA normal group, CSA normal group and Mosapride normal group. After Six days'drug, mice were fasted for 24 h and then administered the last drug and water. One hour later after the last drug, all rats administered 2 mL semi-solid paste. Twenty minutes later, the rats were sacrificed.2 The effect on gastrointestinal motility and brain-gut peptide in chronically stressed and normal ratsAfter seven days'drug, Gastric emptying and intestinal propulsion test were done.The blood was collected by a 10 ml centrifuge tube from femoral artery. Serum separator tubes were used to collecte and allowed to clot for 2 hours at room temperature. Serum was removed and stored at-80 ? after the blood has been centrifuged 15min in 3500 rpm. Intestine and brain were immediately removed from an environment with a low temperature at stored in liquid nitrogen.The contents of MTL and SP in serum of rats was detected by Elisa method. The expression of CCK mRNA in intestine and brain were detected by RT-PCR method.And the data were processed statistically.3 The effect of the rats gastrointestinal smooth muscle in vitroRats were fasted for 24 h,and then the rats were sacrificed.Intestine and stomach were immediately removed from the abdominal cavity,to prepare the isolated rat gastrointestinal smooth muscle samples.The changes of gastrointestinal smooth muscle tension was observed in different concentration of SA and CSA decoction before and after dosing.Results:1 Gastric residue and intestinal propulsion rates after treatmentIn our study, we can see that the gastric residue rate was significantly lower in control group than in CSA normal group (**P< 0.01). The intestinal propulsion rate was significantly lower in control group than in other groups (**p< 0.01 or*P< 0.05). The gastric residue rate and intestinal propulsion rate were significantly higher in SA normal group than in CSA normal group(?P< 0.01 or ?P< 0.05). The gastric residue rate and intestinal propulsion rate were significantly higher in SA group than in CSA group(?P<0.05).2 Serum MTL and SP level rats after treatmentThe serum MTL was significantly lower in control group than in CSA normal group(**P< 0.01). The serum MTL and SP was significantly lower in SA normal group than in CSA normal group (?P< 0.05). And the expression level of MTL in the serum was significantly higher in CSA group than in SA group (?P< 0.05). MTL, SP was reduced in in the serum of model group compared with that of the control, SA, CSA group (**P< 0.01 or*P< 0.05). And the expression level of SP in the serum was significantly higher in CSA group than in SA group (?P< 0.05).3 The CCK expression level in the small intestine and hypothalamus after treatmentThe CCK expression level in the small intestine and the hypothalamus were significantly lower in CSA normal group and SA normal group than in control group (**p< 0.01 or ?P<0.05). There was no significant difference between CSA normal group and SA normal group. The CCK expression level in the small intestine was significantly lower in CSA model group, AS model group than in control group (**P< 0.01). And the expression level of CCK in the small intestine was significantly higher in CSA model group than in SA model group (?P< 0.05). The CCK expression level in the small intestine and hypothalamus were significantly lower in CSA model group, SA model group than in control group (**P< 0.01 or ?P< 0.05). And the CCK expression level was higher in the hypothalamus than in the small intestine.4 The effect on the rats gastrointestinal smooth muscle in vitroBoth SA and CSA could increase the rat stomach fundus smooth muscle tension, and when the final concentration was 10-4,10-3.5,10-3,10-2.5,10-1.5 g/ml, the smooth muscle tension was significantly higher in CSA group than in SA group (P<0.05).Which shows that CSA was more able to increase the smooth muscle tension than Arecae Semen. Both SA and CSA could increase the rat small intestine smooth muscle tension, and when the final concentration was 10-3,10-2.5,10-1.5 g/ml, the smooth muscle tension was significantly higher in CSA group than in SA group (P<0.05 or P<0.01). When the final concentration was 10-4O,10-3.5 g/ml, there was no significant difference between CSA and SA.Conclusions:The model of chronic stress in experiment had FD pathophysiologic features. CSA was more able to increase gastrointestinal motility, Serum MTL, SP level and reduce the CCK expression level in chronically stressed rats, increase the rat small intestine and stomach fundus smooth muscle tension. Which maybe are one of "CSA can be more effective on gastrointestinal motor function" mechanisms.