Preparation And Characterzation Novel Antibacterial Scaffolds For Bone Repair

In the past few years, the increasing number of bone defects caused by disease, trauma, congenital defects and the population aging situation has created an urgent request for bone substitutes to regenerate bones tissues. Although autografts(from the patient) together with allografts(from a donor) are considered as the gold standard for regeneration of some simple and non-load-bearing bone defects, limited supply, donor site morbidity, prolonged recovery, and pathogen transfer restrict their clinic applications. Furthermore, the allogenic and autologous grafts are powerless for regeneration of many serious defects, such as critical-sized bone defects. Since its first definition by Vacanti in 1997, the principle of tissue engineering has been rapidly used to design artificial transplantable materials which, alone or combinedwith bioactive clues, are able to guide bone regeneration, providing a possible alternative to further enhance bone regeneration.Tissue engineering scaffolds combining biomineral and natural polymer are prospective candidate for bone repair materials. In this work, biocompatibility and antibacterial scaffolds Alg/HACC/OSP and PCL/HACC/OSP were prepared by freeze drying. SEM, FT-IR, mechanical test and BET surface area were applied to characterize the prepared scaffolds. The swelling behavior, biomineralization, In vitro biodegradation, protein adsorption, antibacterial test, cytotoxicity, and Alkaline phosphatase(ALP) activity test were also investigated.The prepared scaffolds hold a highly porous and interconnected pore structure with pore size around 20 to 200?m suitable for cell in growth. The addition of oyster shell powder in the scaffolds improved their compressive strength, specific surface areas, and protein adsorption capacity, and also controlled the swelling behavior. Both the scaffolds were biodegradable; the degradation was decreased with increasing of OSP content in the scaffolds, while the biominerability was improved. Meanwhile the prepared scaffolds exhibited antibacterial activity toward E.coli and S.aureus. There was no significant cytotoxicity effect of the prepared scaffolds towards MC3T3-E1, HOS, and MG-63 cells and hold a well ALP activity. Therefore, these results suggest that the Alg/HACC/OSP scaffold and PCL/HACC/OSP scaffold is a prospective candidate for bone repair materials.