| Object:Glioma is the most common primary malignant tumor in the central nervous system and has become a serious threat to human life and health. At present, a comprehensive treatment based on surgery is takenin the clinic, and the standard therapeutic regimen for glioma also includes the postoperative adjuvant radiotherapy and chemotherapy [1]. Although the patients' survival time was prolonged, invasive growth and resistance for therapy of glioma cells result in recurrence even after active treatment [2]. In recent years, with the development of molecular biology technology,biological therapy has beena hot treatment project. Ki-67,a large molecular protein which consists of two polypeptide chains whose molecular weights are 345 k D and 395 k D,respectively,is attributed to one kind of nonhistones. It is a nuclear antigen specifically expressedin proliferative cells, which is closely related to the cell cycle.Ki-67 is expressed in each period of proliferation but do not exsist in quiescent cells, so the synthesis level can reflect the level of cell proliferation. Therefore, it is one of the most widely used indicator to evaluate tumor proliferation activity in current and has extremely important significance to determine the tumor malignant degree and prognosis [3]. Cyclin D1 is overexpressed in a variety of malignant tumors, which is confirmed as the Bcl-1proto-oncogene, is closely related to the occurrence and development of tumor. Although the significance of the overexpression of Cyclin D1 in tumors has not yet been fully confirmed,most scholars believe that it is an important carcinogenic factor [4]. Toll-like receptors(TLRs)are an important class of pattern recognition receptors that found in recent years. They can activate innate immunoreactions via the recognition of pathogen associated molecular patterns and signal transduction induced by some endogenous ligands. The release of inflammatory mediators involved in the occurrence of a variety of human immune related diseases [5]. The expression of TLR4 is not only associated with tumor malignant degree and prognosis but also inhibits immune responses through a variety of ways,promoting the development of cancer [5-9].So TLR4 has become potential target for biological treatment of glioma [10-15]. But the impacts of TLR2 on cancers have not been reported in China.Methods:72 cases of paraffin embedded specimens of glioma tissues in the Department of Neurosurgery of the Affiliated Hospital of Hebei University from January 2014 to January2016 were selected, including 35 males and 37 females, aged from22 to 80 years old, with an average age of 51.4. According to the criteria of WHO 2007 central nervous system tumor classification: there were 49 cases of astrocytoma, 8 cases of small dendritic cell tumor and 15 cases of glioblastoma. Pathological grades: there were 40 cases in I-II grade, 20 cases in III grade and 12 cases in IV grade. The control group was 8 cases of normal brain tissues(NB) in patients with brain injury during the same period.Results:Immunohistochemical results showed that the positive expressionsof Ki-67 and Cyclin D1 in 72 cases of brain glioma were 60.78% and 64.71%, respectively, and neither expressed in normal brain tissues. There were significant differences between the two groups in the glioma and normal brain tissues(P<0.01) and the positive expression rates were positively correlated with the pathological grades of gliomas(r=0.48, P<0.01). The expressions of TLR2 in the lower grade group(I, II) and the highgrade group(III, IV) were statistically significant(X2=24.01, P<0.01). The expression of TLR2 was positively correlated with pathological grade of brain glioma(r=0.46 P<0.01). The expressions of Cyclin D1 in the lower grade group(I, II) and the highgrade group(III, IV) were statistically significant(X2=10.72, P<0.01). There were significant differencesbetween proliferation indexes of Ki-67 at all levels of glioma tissues(F=1.00, P<0.01). In addition, TLR2 was positively correlated with the expression of Cyclin D1(r=0.75, P<0.01). The expression of TLR2 was positively correlated with Ki-67(r=0.983, P<0.05).Conclusion:1 TLR2, Cyclin D1 and Ki-67 were only expressed in brain gliomas, but not expressed in normal brain tissues. And their expressions were positively correlated with the pathological grades of gliomas.2 The expression of TLR2 was positively correlated with that of Ki-67, which revealed that the expression of TLR2 was related to the invasion of gliomas.3 The expression of TLR2 was positively correlated with that of Cyclin D1, indicating that TLR2 and its cell signal transduction pathways involve in the abnormal proliferation of gliomas. |
PDF Full Text Request