The Promotion Role Of EPO On The Proliferation Of Glioma Through Receptor ?cR

ObjectiveThis study aims to explore the effect of erythropoietin on the proliferation of glioma by binding to PcR.MethodIn this study,we detected the expression of EPO and ?cR in human glioma tissues by immunohistochemistry.We used lentivirus transfection technology to silence ?cR gene in U87 glioma cells and established a model of glioma xenograft in nude mice with the U87 cells,then observed the tumor formation.The U87 cells were cultured in vitro and divided into three groups:control group,EPO treatment group,?cR-shRNA+EPO treatment group.The expression of CyclinD1 was detected by Western blot,the proliferative activity was tested by Cell Counting Kit-8 assay and Clone formation,respectively.ResultsThe expression of EPO was increased in the high grade glioma group compared to the low grade glioma group(P=0.0016)and the PcR expression in the high grade group was also higher than that in the low grade group(P=0.0028).During the subcutaneous tumorigenesis test,the expression of CyclinD1 was higher in the EPO treatment group(P=0.0056),and the tumor grew faster than the control group(Pweight=0.0026,Pvolume=0.0030).The tumor growth rate was significantly lower in the ?cR-shRNA+EPO treatment group than that in the EPO treatment group(Pweight=0.0011,Pvolume=0.0010),the expression of CyclinD1 was also lower in the ?cR-shRNA+EPO treatment group than that in the EPO treatment group(P=0.0044).Compared with the control group,the cell proliferation of the EPO treatment group was obviously increased(P3d/5d=0.0200/0.0280,PColone=0.0320),and the expression of CyclinD1 was significantly enhanced(P=0.0108).Compared with the EPO treatment group,the expression of CyclinD1(P=0.0095)and the proliferation of glioma cells(P3d/5d=0.0079/0.0090,PColone=0.0080)were significantly reduced in the ?cR-shRNA+EPO treatment group.ConclusionOur results indicated that Erythropoietin may significantly promote the proliferation of glioma through ?cR.