| The mammalian Toll-like receptors (TLRs) are homologues of Drosophila Toll and conserved sequence during species evolution. Twelve members in TLR family have been found since the first TLR molecular was identified in 1997. These conserved receptors share high homology with Drosophila Toll and recognize a wide variety of pathogenic microorganism and their component. As the pattern recognition receptors, TLRs can mediate the activation of innate immunity, and also link to antigen-specific acquired immunity. Besides anti-infection immunity, TLR family also plays an important role in autoallergic disease, hypersensitivity reaction, graft rejection, ischemical reperfusion injury and tumor immunity.TLR2 is the receptor for a variety of microbial ligands, including peptidoglycan from gram-positive bacteria, zymosan from yeast, ara-lipoarabinomannan (araLAM) and lipopeptide from mycobacterial. In this research, expression of TLR2 was detected in different murine tumor cell lines. Meantime recombinant plasmid pcDNA3.1(-)-mTLR2(-) including TLR2 anti-sense was constructed and was transfected into NS-1 cell line leading to expression of TLR2 in NS-1 cell line down regulation. And tumor model was established in mice to evaluate the effect of TLR2 on tumor occurance.Part â… Detection the expression of TLR2 in murine tumor cell lines and construction of recombinant plasmid pcDNA3.1(-)-mTLR2(-)Four primers from P1 to P4 (P1, P2 for TLR2 and P3, P4 for 6-actin) were... |
PDF Full Text Request