A Comparative Study Of Different Preparations Of Wendan Decoction On The Treatment Of Hot Sputum Interference Insomnia

Objective: Based on the traditional decoction,Comparative study on HPLC-DAD fingerprint of 2 kinds of modern Wen Dan Decoction by its n-butanol extract.To establish the process and rationality evaluation method which based on the characteristic of chemical composition fingerprint.Preparate Wen Dan Detecion which is used to treat Phlegm heat disturbance type insomnia as a classical prescription,then Study on preparation technology, quality standard and effect of drug action.Methods: Using the Kromasil C18 column(250 mm* 4.6 mm,5?m), the mobile phase was acetonitrile- 0.3%phosphoric acid gradient elution, the flow rate was 1 m L·min-1,the detection wavelength was 237 nm, the column temperature was 25?;Research the above 3 kinds of preparation technology of fingerprint by using the method of finerprint detection in contrast;the 3 kinds of different preparation technology of n-butanol extract of HPLC fingerprint was evaluated by using "traditional Chinese medicine chromatographic fingerprint similarity evaluation system" 2004 edition and investigate the relative retention time and relative peak area of each characteristic peak.Preparate Wen Dan traditional decoction refer to the method of literature. The forming process of granules was investigated by the flow rate, moisture absorption and auxiliary materials ratio. Using TLC method to identify citrus, tangerine peel, tuckahoe and liquoric, 6- gingerol, bamboo shavings. Using HPLC to determine the content of hesperidin, naringin, liquiritin, glycyrrhizic acid, 6-gingerol.Investigate the The sleep effects of different form of Wendan Decoction by the Sedative experiment?Sleep experiment with pentobarbital sodium.Results: Having Preparated 3 different form of Wen Dan Detecion and established it`s HPLC fingerprint in vitro. The Common peak number of traditional decoction?morden decoction ?Granule are 10?13?10.there are 10 common peaks among 3 preparations.5peaks were identified respectively,naringin,hesperidin, liquiritin ammonium glycyrrhizinate and 6- gingerol. Compare the HPLC fingerprint similarities in 3 kinds of preparation's n-butanol extract were more than 0.85.The RSD of relative retention time and relative peak area lower than 2% at common peaks. Spots were obtained from the test solutions and without interference.The content of Liquiritin hesperidin, naringin, Ammonium Glycyrrhizinate, 6- Gingerol respectively as no less than 0.85?10.16?12.55?3.06 mg/g.The results of Sedation and sleep efftct with pentobarbital sodium in different groups of drug Administration have significant difference. There was no significant difference in pharmacological experiment results of the three dosage forms under the same dosageConclusion: Above data description: Considering the Material basis and pharmacological action, Wen Dan decoction with different preparation technology Has certain equivalence Under specific experimental conditions. Wen Dan Decoction granule have stable process which quality control methods are rigorous?reasonable and repeatable.