| Objective: Through the observation after administration of pregnancy rats live birth number, abortion, abortion rate to assess effects of cuscuta ginseng tocolysis particles tocolysis efficacy and study the dodder ginseng tocolysis particles on the morphological changes of the pregnant rat uterine decidua, and through the detection to the concentration of each pregnant rat uterine decidual leukocyte mediated IL-10,tumor necrosis factor TNF-α after administration, from the perspective of immunology preliminary study dodder ginseng tocolysis particles tocolysis mechanism, dodder ginseng tocolysis granule in the treatment of threatened abortion provide scientific experimental basis. Methods:1. Grouping: female rats and male rats by 2: 1 mated, daily early morning 8 when to female mice vaginal smear, under a microscope, found a large number of sperm or inside the vagina of the female rats were found with a vaginal plug them as the first day of pregnancy. Random pregnant rats set into five groups: a normal pregnancy group, B model group, progesterone group C and D dodder ginseng tocolysis granule in high dose group, dodder ginseng tocolysis granule low dose group, 10 rats in each group2.Administration and modeling: from the 1-11 days of pregnancy and normal pregnancy group, model group of each pregnant rats gavaged with saline 2ml / D, 1 times a day; progesterone group the next day intramuscular injection of progesterone(6mg / kg) 1, 6 times in total; dodder ginseng tocolysis granules in high, low dose group of pregnant rats were given service cuscuta ginseng tocolysis particles 35.20g/kg, 8.80g/kg, 1 times a day. Pregnant rats(normal pregnancy group except) on the tenth day of pregnancy fed with Mifepristone Tablets, using 4mg/kg to establish the rat model of threatened abortion.3.Specimen collection and detection: from gestation day 12 pregnant rats of each group were sacrificed, dissected immediately record bed points, the number of live births, the number of abortions and calculation abortion rate; take the side of decidual tissue made homogenate, supernatant, at 80 DEG C preservation, by enzyme linked immunosorbent assay measured IL-10, TNF-α content; on the other side of the uterine decidua conventional embedded and fixed, he staining, section, under the light microscope observation the morphologic changes of decidual tissue. Results:(1)The pregnant rats in model group the number of live births was significantly lower than that of normal pregnancy group, by statistical treatment, there was a significant difference(P < 0.05), abortion, abortion rate was significantly higher than that of normal pregnancy group, there was significant difference(P < 0.05) compared that threatened abortion rat model is successful. Dodder ginseng tocolysis granules in high, low dose group, progesterone group of pregnant rats the number of live births was significantly higher than that in the model group, respectively with the model group compared with the presence of significant difference(P < 0.05), dodder ginseng tocolysis granules in high, low dose group, progesterone group of pregnant rats abortion, abortion rate was significantly lower than that in the model group, respectively, and the model group compared to the existence of significant differences(P < 0.05), was statistically significant. The progesterone group the number of live births less than high dose group, there were significant differences(P < 0.05) compared with and was statistically significant. The number of abortion, abortion rate was significantly higher than that of Cuscuta ginseng tocolysis particles in high dose group, by statistical treatment there were significant differences(P < 0.05). Rate of progesterone group of live birth number, abortion, abortion respectively, compared with the low dose group and the statistical treatment no significant difference(P > 0.05). In the low dose group, the number of live births, the number of abortion and abortion rate were significantly different compared with the high dose group(P < 0.05). Dodder ginseng tocolysis particles, progesterone can improve the threatened abortion in pregnant rats the number of live births, reduce the number of abortion and abortion rate, miscarriage and dodder ginseng tocolysis particles high dose group spuc curative effect is better than that of progesterone group.(2) Compared to its structure and glandular morphology was incomplete, nucleus cytoplasm ratio increases, partial villous structure of cellulose, visible marked hyperemia, part focal apoptosis and necrosis, and invasion and superficial muscular layer and normal pregnancy normal pregnant rats uterine decidual tissue structure and morphology in glands, villi structure is clear, no obvious congestion, no marked hyperkeratosis and glandular differentiation trend model of pregnant rats decidual tissue morphology and normal pregnancy group. Dodder ginseng tocolysis granules in high, low dose group, progesterone group of pregnant rats decidual tissue morphology and model group compared to the three groups of uterus in varying degrees of degenerative changes, but the decidual tissue structure roughly normal, gland morphology basically complete, between stromal cells did not see obvious cornification and glandular differentiation trend, can be seen scattered in small foci of hemorrhage, a small amount of inflammatory cell infiltration, this three group of decidual tissue degeneration degree of light, significantly better than the model group.(3) the concentration of TNF-α in the uterus of the model group was significantly higher than that in the normal pregnancy group, and there was a very significant difference(P <0.01),Compared with the model group, the pregnant rat uterine decidual tissue concentration of TNF-α and dodder ginseng tocolysis particles in high dose group, dodder ginseng Antai granule low dose group and progesterone group pairwise, there is a very significant difference(P <0.01)Progesterone group, dodder ginseng Antai granule low dose group of pregnant rats TNF-α concentrations were higher than that of Cuscuta ginseng tocolysis particles in high dose group, compared with dodder ginseng tocolysis particles in high dose group, there are significant differences(P < 0.05). Comparison of the serum level of TNF-α and dodder ginseng tocolysis particles low-dose group progesterone group of pregnant rats, no significant difference(P > 0.05). Experimental results show that the dodder ginseng tocolysis particles and progesterone can reduce the concentration of TNF-α in threatened abortion rat uterine decidua and dodder ginseng tocolysis particles high dose group curative effect than progesterone group. IL-10 concentration(4) model group of pregnant rats was significantly lower than that of normal pregnancy group, there is a very significant difference(P < 0.01) between compared, progesterone group, dodder ginseng tocolysis granules in high, concentrations of IL-10 in low dose group was significantly higher than the concentration of IL-10 in model group, presence of very significant difference(P < 0.01); progesterone group, dodder ginseng tocolysis particles low dose group of IL-10 concentration lower than the dodder ginseng tocolysis particles in high dose group, respectively compared with dodder ginseng tocolysis particles in high dose group, compared to significant differences(P < 0.05). The progesterone group of TNF-α concentration and dodder ginseng tocolysis particles low dose group compared no significant difference(P > 0.05). The experimental results show that dodder ginseng tocolysis particles and progesterone can be elevated IL-10 concentration of threatened abortion rat uterine decidua and dodder ginseng tocolysis particles high dose group efficacy and in the progesterone group. Conclusion: the method is simple and easy to operate, and has high success rate, and it is widely used at present. Dodder ginseng tocolysis particles, progesterone can improve threatened abortion model rats the number of live births, reduce the number of abortions, the rate of abortion, tocolysis efficacy, in dodder ginseng tocolysis particles high dose group tocolysis efficacy best; dodder ginseng tocolysis granule can improve threatened abortion model rats uterine decidua tissue morphological structure; dodder ginseng tocolysis particles, progesterone can reduce TNF-α concentration of threatened abortion model rats, improve IL-10 concentration, the effect to dodder ginseng tocolysis particles high dose group tocolysis efficacy best. |
PDF Full Text Request