Research On The Relationship Between Phthalates Exposure Levels In Adults And Changed Body Mass Index In Northern Capital City

Objective:Toxicology studies have shown that phthalates(Phthalate acid esters, PAEs) in the environment can cause increased body weight of mice, but these findings are still needed to be corroborated through epidemiological research data on population. Currently, we have less appropriate epidemiological studies, and can not reach consistent conclusions. In this paper, Cross-sectional study was conducted in a hospital-based population,we preliminary study the relationship between PAEs exposure and adult BMI and other obesity-related indicators changes.Contents and Methods:We selected those healthy adults who participated in the examination of health at the Third Hospital of Hebei Medical University as research subjects,the research lasted from October,2014 to June,2015. After completing demographic information questionnaire(such as height, weight, age, etc.),we collected their blood samples in vein. Part of the blood samples was used to detect biochemical markers such as Total Cholesterol, Triglycerides, Fasting Glucose, while the remainder was examined by Gas Chromatography tandem Mass Spectrometry(GC-MS) to determine 16 common PAEs levels in serum.When data collection was completed, we took a Spearman rank correlation analysis for nine PAEs which had a higher detection rate(more than 30%),Principal Component Analysis was employed to reduce the dimension for the purpose to extract several main ingredients. Finally, In order to research the links between exposures and effects, a Generalized Linear Regression model was applied to explore the correlation between the respective PAEs monomer and obesity-related indexes. Eventually, we obtained values of responding regression coefficient ? and their 95% confidence intervals.Results:1 Demographic data We had 270 objects involved,Age ranged from 18 to 45 years, and the mean age was 26.93 ± 5.67 years. Among them 225 were males(83.3%),while 45 were women(16.7%).2 Concentrations of PAEs and their detection rates16 kinds of PAEs in serum was detected, among them Di-iso-butyl ortho-phthalate(DIBP) and Di-n-butyl ortho-phthalate(DBP) had the highest detection rate(100%) and the median concentrations were 0.0498?g/ml,0.1081?g/ml;For comparison, the detection rate of Dimethyl ortho-phthalate(DMP),Bis(4-methyl-2-pentyl) phthalate(BMPP), Di-n-octylortho-phthalate(DNOP), Bis(2-ethylhexyl) ortho-phthalate(DEHP)monomer was more than50%, while the remaining monomer were under detection rate.3 Results of Body Mass Index and other Obesity-related effective indicators:Values of Body Mass Index, serum Tiglyceride levels, Total Cholesterol and Fasting Glucose concentrations were 23.51 ± 3.84 kg/m2,1.95± 0.28mmol/L, 4.52 ± 0.94 mmol/L, 5.46 ± 0.77 mmol/L.4 Correlation analysis and Principal Component Analysis Spearman rank correlation analysis showed that nine kinds of PAEs which had a higher detection rate(over 30%) were statistically significant correlated between each other. Principal component analysis was adopted and as a result we acquired three principal components which accounting for64.42% of the total. Among them, the first component had a contribution of28.54%, comprising DIBP, DBP, DCHP and DNOP; the second component accounted for 20.96% which included BMPP, Diisononyl ortho-phthalate(DINP) and Benzyl-n-butyl ortho-phthalate(BBP); The 3rd main component contained DEHP and DMP and held a ratio of 14.91 percent.5 Relationships for BMI indexs and other indicators with PAEs exposure Relationship between BMI and DIBP was positively correlated(P-trend<0.05). Compared with the low level group, the highest exposure group with a BMI index increased by 1.246 kg/m2(95% CI: 0.004, 2.488); when TG was log-transformed, DEHP and DNOP exposure was found to have a significantly positive correlation(P-trend <0.05) with TG. Compared with the reference group, DEHP and DNOP which was at the highest level group had a overwhelming TG concentration by increasing 0.082 mmol/L(95% CI: 0.005,0.160) and 0.089mmol/L(95% CI: 0.001, 0.178); It was amazing to get no relationships between TC,GLU and PAEs exposure.Conclusion:DIBP exposure levels has a positive correlation with Body Mass Index;Similarly, there exists a strongly positive association between DEHP as well as DNOP exposure levels and serum Triglyceride concentrations. At last, PAEs exposure may contribute to the increased Body Mass Index and Triglyceride levels.