Analysis Of The Index Component Of Calonyction Muriatum (Linn) G. Don. And Extracting Process Optimization For Cancer Pain Relieving Granules

Cancer pain is a kind of chronic pain with unique and complex mechanisms. Its occurrence is related to peripheral afferent nerve sensitization and central sensitization. At the early stage, cancer pain was caused by pain substance and sensitization of primary afferent-based that induced by the tumor cells, inflammatory cells and the continued activation of osteoclasts. At the late stage, nerve compression and damage caused by tumor growth was account for the genesis of cancer pain.Currently, although curative effect of western medicine treatment of cancer pain is accurate, the toxic side effects, strongly dependence, high cost of treatment, irreversible neurological damage etc. Chinese traditional medicine play an important role in local treatment and systemic regulation, also has the advantages of little dosage, less adverse reactions, curative effect, easily accepted and extensive clinical application.The compound preparation mentioned in the subject consists of six herbs- Ipomoea Hardwickii Hemsl, Radix paeoniae Alba, Rhizoma Corydalis etc. One of the components of Ipomoea Hardwickii Hemsl was determined by UV-visible absorption spectrum, proton nuclear magnetic resonance spectra(1H NMR), carbon nuclear magnetic resonance spectra(13C NMR). The component of Ipomoea Hardwickii Hemsl was ipalbine through the analysis of spectral data, this date was found to agree with the literature value.According to the active ingredients and pharmacological functions of the pharmaceuticals in compound prescription, three preliminary processes for extraction technology was obtained. Analgesic action of the three processes for extraction technology was evaluated through the hot plate test and the acetic acid induced wringing test in mice. Toxic properties of the three processes were evaluated through the acute toxicity test. The results showed that the three processes all have significant analgesic action, the second process has a more significant and durable analgesic action with dose-effect relationship on the central and peripheral pain compared with the others. The degrees of toxicity of the obtained preparations are of no significant differences for the three processes.The orthogonal experiment based on ethanol concentration, ethanol amount, extraction time, water amount, density of paste was designed and the single- factors experiment of filter method, concentrated temperature was investigated to optimize the second process. The optimum process with efficiency, economic, reasonable and feasible feature was established. The optimum condition of the extracting process was as follows: ten and eight times amount of 50% ethanol solution were used to extract the active ingredient twice from the three kinds of herbs include Calonyction muriatum(Linn) G. Don. and obtained the ethanol-extracting-solution; twelve and eight times amount of water were used to extract active ingredients of the other three kinds of herbs include white paeony root by decocting twice and each time last for 1 h, then the solution was filtered and concentrated to that with relative density of 1.05 g/mL(60 ?), suitable amount of ethanol was added to obtain a 50% alcohol solution, rested overnight to give the ethanol-deposit- fluid; filtered the ethanol-extracting-solution and the ethanol-deposit-fluid with strainer(80~100 mesh) and concentrated to a relative density of 1.2 g/mL(60 ?), vacuum dried for18 h(60 ?).