Study On Relationship Between Gene Polymorphisms Of LTA4H And Susceptibility To Tuberculosis

Background: Tuberculosis was defined as an infectious disease that cased by Mycobacterium tuberculosis (M.tb). Several factors influence the beginning and severity of the disease, include environmental and host genetic factors. Many genes were indicated to associate with susceptibility of tuberculosis. LTA4H is a key enzyme of AA metabolism pathway which catalyzes the final step in the biosynthesis of leukotriene B4. The level of LTB4regulates the biosynthesis of many cytokines of human. Study of animal model suggests LTA4H influence the susceptibility of Zebrafish larvae to Mm. Therefore, polymorphisms in the gene that encodes the LTA4H may influence the host response to M.tb infection.Objective:Through the study of polymorphisms in LTA4H gene in tuberculosis patients and healthy controls to investigated whether LTA4H single nucleotide polymorphisms (SNPs) are involved Chinese Han population.Methods:200tuberculosis patients and200health controls were enrolled in this study. Polymorphisms and genotype SNPs were detected via sequence. SNPs of the LTA4H gene were analyzed by SNaPshot technique. Allele frequency, genotype frequency, linkage disequilibrium (LD) and haplotypes were analysis suing SHEsis software.Results:Five high frequency SNPs (rs1978331, rs2247570, rs2660898, rs2660845and rs2540474) were chosen by sequence. There were no significant difference of allele frequency and genotype frequency of these SNPs between cases and controls (P>0.05). SNPs rs1978331and rs2660845were strong Linkage Disequilibrium, and haplotype analysis using SHEsis software. Our study revealed4LAT4H haplotypes among tuberculosis patients and health control. Comparie of these haplotypes, the result indicated that there were no significant differences in the haplotype frequency between tuberculosis patients and TB and health control group (P>0.05).Conclusions:LTA4H gene polymorphisms do not appear to influence the susceptibility to tuberculosis. Results suggest that LTA4H gene polymorphisms may not major genetic factors for susceptibility to tuberculosis in Chinese Han population.