CCL5, CCR5 And MIF Gene Polymorphisms And Susceptibility To Tuberculosis

Objective To analyze the relationship between CCL5, CCR5, MIF gene polymorphism and susceptibility of tuberculosis(TB) in Han population of China. Methods A total of 428 TB patients were selected as case group from Beijing Chest Hospital, Capital Medical University between February 2014 and April 2015. 387 healthy volunteers were selected as a control group. Venous blood was collected. The single nucleotide polymorphisms(SNPs)of CCL5 rs2107538?rs2280788, CCR5 rs1799987 and MIF rs2070767 were detected by using competitive allele-specific PCR SNPline program. Using the R language calculated for each locus Hardy-Weinberg equilibrium assess, we also calculated the genotype and allele frequencies of case group and control group were calculated in order to analyze the relationship between CCL5, CCR5, MIF gene polymorphisms and susceptibility of TB. Using Haploview4.2 application software builds CCL5 gene haplotypes and construction diplotypes gene to analyze the correlation with susceptibility to tuberculosis. Using MDR and logistic regression analysis interaction between SNPs. Results The genotype CC, CG, GG and allele C, G frequencies of CCL5 rs2280788 in case group were 77%(330/428), 22%(94/428),1%(4/428) and 88%(754/856), 12%(102/856); in the control group were 76%(295/387), 22%(87/387),1%(5/387) and 87%(677/774), 13%(97/774). There was no statiscally difference in genotype and allele between the two groups(?2 = 0.28, 0.0923, all P values> 0.05). The genotype CC, CT, TT and allele C, T frequencies of CCL5 rs2107538 in case group were 42%(178/428), 46%(196/428), 13%(54/428) and 64%(552/856), 36%(304/856); in the control group were 36%(139/387), 51%(197/387), 13%(51/387)and 61%(475/774), 39%(299/774). There was no statiscally difference in genotype and allele between the two groups(?2 = 2.831, 1.5627, all P values> 0.05). The genotype GG, GA, AA and allele G, A frequencies of CCR5 rs1799987 in case group were 18%(77/428), 57%(245/428), 25%(106/428) and 47%(399/856), 53%(457/856); in the control group were 16%(67/387), 51%(197/387), 33%(128/387) and 41%(321/774), 59%(453/774). There were statiscally differences in genotype and allele between the two groups(?2 =6.8545, 4.1475, all P values<0.05). The genotype CC, CT, TT and allele C, T frequencies of MIF rs2070767 in case group were 38%(162/428), 50%(216/428), 12%(50/428) and 63%(540/856), 37%(316/856); in the control group were 42%(162/387), 47%(180/387), 12%(50/387) and 65%(504/774), 35%(270/774). There was no statiscally difference in genotype and allele between the two groups(?2 = 1.477, 0.6434, all P values> 0.05). There were statiscally differences in haplotypes G-C of CCL5 gene rs2280788 and rs2107538 SNPs between the two groups(OR=4.13, 95%CI: 1.35-16.85). There were statiscally differences in diplotypes CG-CC of CCL5 gene rs2280788 and rs2107538 SNPs between the two groups(OR=12.07, 95%CI:1.8-513.99). Analysis between loci MDR interaction, rs1799987 locus statistically significant. Using logistic we found rs1799987 AA more susceptible than AG, but also more susceptible GG compared with AG. Conclusion There was no significant correlation between CCL5 rs2107538 and rs2280788 polymorphisms and susceptibility of TB. Carrying CCR5 rs1799987 GG genotype and allele G may reduce the risk of TB. There was no significant correlation between MIF rs2070767 polymorphisms and susceptibility of TB. Haplotypes G-C and diplotypes CG-CC of CCL5 gene rs2280788 and rs2107538 SNPs may be a risk factor for TB disease. Using MDR and logistic regression found significant correlation between rs1799987 polymorphisms and susceptibility of TB.