Etiological Investigation Of Canine Parvovirus And Canine Distemper Virus In Changchun Of Jilin Province

With the growth in the living standard,companion animals,such as dogs and cats,have gradually become an important part of people life,and the disease in cats and dogs pose a serious threat to their health.Canine parvovirus and canine distemper virus disease were the most common canine clinical disease Characteristics with Strong infectious,high incidence,high mortality and Worldwide epidemic,caused serious threat to the health of dogs.In this research,the epidemiology and genetic differences of canine distemper virus(CDV)and canine parvovirus(CPV)were investigated in Changchun,and it will be benefit to the further study of etiological investigation and establishment of prevention of CPV and CDV.Etiological investigation for CPV and CDV of 539 dogs in pet hospital located nine different district/county of Changchun in Jilin province from December 2015 to January 2017 was surveyed using the gold rapid detection kit in this research.The results of etiology investigation: In total tested samples,the positive rate of CPV was 28.57%,the positive rate of CDV was 17.25%,CPV+CDV were 3.15%.The highest incidence of CPV was in March for 38.29%,and the lowest incidence was in July,August,September,for 16.13%,16.27% and 16.67%,respectively.The highest incidence of CDV was in March,April,and November,for 21.28%,24.36% and 22.22%,respectively,the lowest incidence was in August for 9.30%.Through the analysis,we found that dogs of 2~4 months have highest incidence rate of CPV at 38.17%,and dogs of 0~2 months have highest incidence rate of CDV at 23.81%.Furthermore,the difference between males(positive rate of CPV was 29.70%,CDV was 17.82%)and females(positive rate of CPV was 27.12%,CDV was 16.53%)was not significant.The incidence of CPV and CDV was highest in Small scale dogs,for 32.99%,19.79%,respectively,and lowest in large scale dogs,for 22.61%,14.78%,respectively.The incidence rate of CPV and CDV for non-immune dogs was highest,followed by unfinished completely immune dogs,the lowest was completely immune dogs.Furthermore,the difference between males(positive rate of CPV was 29.70%,CDV was 17.82%)and females(positive rate of CPV was 27.12%,CDV was 16.53%)was not significant.The incidence of CPV and CDV was highest in Small scale dogs,for 32.99%,19.79%,respectively,and lowest in large scale dogs,for 22.61%,14.78%,respectively.The incidence rate of CPV and CDV for non-immune dogs was highest,followed by unfinished completely immune dogs,the lowest was completely immune dogs.Through the analysis,we proved there was no significant relationship between the incidence of CPV and CDV and region and gender,but was significant with month,age,varieties,origin and immune situation.Genotype distribution of CPV and CDV in Changchun was analyzed based on VP2 gene of CPV and H gene of CDV.The results showed that 5.56% tested samples were CPV-2,61.11% were CPV-2a,30.56% were CPV-2b,2.78% were CPV-2c in 36 random samples from CPV positive samples,and 91.67% tested samples were belong to the type of Asia-1 of CDV,8.33% were belong to the type of America in 12 random samples from CDV positive samples.In conclusion,the morbidity of CPV of pet hospital dogs in Changchun was higher than the morbidity of CDV,where the morbidity of 0~4 months' dogs was highest,and mainly in March,April,and November,Susceptible dog groups mainly was small to medium sized dogs,purebred dogs and non-immune dogs.In addition,the genotype of CPV in Changchun in this investigation mainly was CPV-2a,followed by CPV-2b,and the genotype of CDV mainly was belong to the type of Asia-1.