Clinic Diagnosis, Treatment And The Pathogens Isolation And Identification Of Canine Distemper And Parvovirus Disease

Canine distemper and parvovirus disease are severesly lethal diseases. Canine distemper is characterizedby Bronchitis, catarrhal pneumonia and severe enteritis; while canine parvovirus disease is mainlycharacterized by Hemorrhagic enteritis or nonsuppurative myocarditis. They are most harmful for puppies,especially at age of2~4months, lethallty rate by which could reach to70%. Based on15clinical cases ofthese two diseases (12cases of parvovirus disease,3cases of canine distemper), the diagnosis andtreatment were analyised and the isolation and identification of the pathohens were conducted to lay asicnetific base for the better treatment and the preparation of vaccine for prevention canine distemper andparvovirus diseases.By means of clinic observation diagnosing kits12dogs were diagnosed as canine parvovirus disease and3dogs were done as canine distemper. Dogs are Divided into groups of canine distemper group andparvovirus group.Breathing heart rate, body temperature of canine distemper group are Higher thannormal,Respectively39.00±0.58times/min127.33±1.45times/min,39.80±0.30℃.All of they Aredominated airway inflammation,Clinical manifestations are the nasal cavity surrounded by yellow purulentnasal discharge,there are a lot of purulent secretion Canthus, with cough. Breathing, heart rate, bodytemperature of parvovirus group are within normal,Respectively21.50±1.50times/min，119.00±0.58times/min，38.40±0.10℃，Clinical symptoms are characterized by vomiting, diarrhea,and bloody row inAdvanced disease. using intravenous infusion therapy of monoclonal antibodies,Interferon subcutaneousinjection,symptomatic treatment on Canine distemper canine distemper group. detection of Colloidal goldtest strip for the antigen of CDV and CPV. using intravenous infusion therapy of monoclonalantibodies,Interferon subcutaneous injection,symptomatic treatment on Canine distemper canine distempergroup. After16~20days2dogs fully recovered,one dog Appeared neurological symptoms in the11thdays,and died one week later.therapy of Canine parvovirus group by using cardiac rehydration,anti-bacterial antiinflammatory, anti-viral treatment principles After4~10days10dogs fully recovered,Serious bloody phenomenonin Appeared in two dogs and died soon by Drug hemostasis is invalid.The clinic samples of dead dogs were collected and inoculated to Vero cells using synchronous culturemethod. The RT-PCR was used to identify the viruses with test of50%tissue-culture infective dose(TCID50). The infected cells showed significant cytopathy of cell shrinkage and loss, in which the specificfragments of CDV genes were amplified separately.335bp for CDV gene. The TCID50of both pathogenswas10-3.00/0.1mL. In order to isolate one local popular CPV strain, to Inoculated clinical samples of dogvaccination in the MDCK cells, by PCR technology, virus drops (TCID50) detection, for the identificationand determination of virulence of the virus. Results: the infection of MDCK cells there is an obviouspathological changes, characterized by cell falls off, round; specificity fragment were amplified both Intissue and cultured cell strains of CPV virus gene fragments, fragment length is374bp; TCID50is10-3.67/0.1mL.It is showed that distemper intravenous infusion of monoclonal antibodies and symptomatic treatmentmethod is feasible. The pathogens isolated and identified in the study could be used as the strain to developthe vaccines for preventing CPV and CDV.