Protective Effect Of Probiotics Against Eimeria Tenella Coccidiosis In Chickens

Chicken coccidiosis is caused by one or several kinds of coccidiosis in chicken intestinal epithelial cells,which can lead to the destruction of intestinal tissue and intestinal flora.Chicken coccidiosis is widespread and do great harzard to powtry industry in the world,intensive poultry industry is most common,causing huge economic losses to the poultry industry.According to statistics,the world poultry coccidiosis cause huge economic losses,up to $300 million each year.Eimeria tenella infection,commonly known as cecal coccidiosis,is the most serious coccidiosis.With the continuous use of chemical drugs,the drug residues in poultry and the problem of drug resistance has become the focus of attention.Therefore,it is urgent to find an effective,safe and low toxic anti coccidiosis mechod.In this study,microoganisms in caecum were to The probiotic bacteria,Bacillus subtilis,Lactobacillus and yeast were used to investigate the protective effect of probiotics on E.tenella infected chickens.The results are as following experiments:(1)Sequencing of microorganisms isolated from caecum of chicken infected with E.tenellaTo establish the E.tenella infection model,the chickens were divided into two groups,that is infectied group and control group,The cecal tissue were collected,Then the cecum microbial genome DNA were extracted,then sequenced.The experimental results showed that in the analysis of the diversity of Alpha in infection group compared with the control group,infectioned group ACE were infection increased 16.86,Chao1 were increased 18.4,Simpson was increased 0.01 in infected group compared with the contry group E.tenella has great influence on the microbial community,changes different microbial bacteria in the phylum and genus level,in the analysis of the diversity of Beta in the control group,The sample space between distance and space between the infectied group and control group were changed by the Beta analysis.(2)Protective effect of probiotics on E.tenella infected chickensE.tenella infection were model of chicken established,The clinical symptoms,stool score,survival rate,growth rate were determined,To study the protective effect of probiotics on chickens infected with E.tenella.The results showed that the addition of probiotics can reduce coccidial infection of chicken blood stool score,significantly reduce the mortality of chickens infecte with E.tenella,improve the growth rate of chicken.The probiotic anti coccidiosis index was 137.The results showed that probiotics can be combined with other feed additives in the prevention and treatment of clinical coccidiosis.(3)Effects of probiotics on the structure and golbet cells of cecum in chickens infected with E.tenellaThe established of E.tenella coccidian vaccination test model,in seventh days,from chicken cecal tissue were collected after weighing,Pathological changes of cecal tissue pathology were observe by using of HE staining,Goblet cells and glycogen and mucin were observe by using of PAS and AB-PAS staining.The experimental results showed that,The of caecum was damaged with the E.tenella infection the treatment of probiotics the damaged was allivated strwwtue of caecum.After infection with Eimeria tenella,the cecal mucosa was destroyed,and a lot of glycogen and mucus were lost.Conclusion: The divesity of microorgavisw in cacum was changed with the infection E.tenella.The addition of probiotics in the treatment of Eimeria tenella infection can effectively reduce the score of chicken feces,improve the weight gain rate of coccidiosis infected chickens,and reduce the damage of intestinal mucosa and intestinal glands.It can significantly improve the survival rate of infected chickens,improve the intestinal mucosal immune function,and play a role in anti coccidiosis.