Effect Of Different Time Of Application Nitrogen On Carbon And Nitrogen Metabolism Of Wine Grapes By Drip Irrigation

In this experiment, 10-year-old 'Cabernet' grapes was used as test material, research on the effect of leaves with the carbon and nitrogen metabolism enzyme activity, sugar and starch content of leaves and endogenous hormone levels, and the yield and quality of fruit harvest, to determine the most appropriate brewing viticulture nitrogen period in Hexi Corridor. The main conclusions are as follows:1.Applying the nitrogen fertilizer in emergence period and the new shoots flourishing period, leaves had the highest Pn before 5 d flowering and 20 d after flowering. In flowering stage and the first stage of fruit expansion apply the nitrogen, the Fv'/Fm',Fv/Fm,?PS?and q P in leaves all reached the highest value 50 d after flowering. This indicates that the three stages of nitrogen application increased the rate of light energy capture and conversion; Apply nitrogen fertilizer in the new shoots flourishing stage and in flowering stage, the chlorophyll content was at a high level in different periods.2.120 d after flowering, nitrogen treat current year new roots in the flowering period, the content of organic carbon is lowest, applying the nitrogen fertilizer in the treatment of the new root of the year in the first stage of fruit enlargement, the carbon content is highest; During the first stage of fruit enlargement and the sublateral shoot growth stage, the change of organic carbon content in the whole growth period could be changed with the application of nitrogen fertilizer.3.In flowering stage and the first stage of fruit expansion, application of nitrogen fertilizer could significantly increase the sucrose content in all leaves of 20 d after flowering, and it has significant differences with other treatments; Applying the nitrogen in sprouting period and new shoots flourishing period, the contents of glucose and fructose in the leaves were higher than other treatments during the whole growth period. Applying the nitrogen in flowering stage, the first stage of fruit expansion, the growth stage and the second stage of fruit expansion. In the new shoot growing, flowering and side shoot growth period, the leaf SS activity at 20 d and 50 d after flowering was almost the same with applying nitrogen fertilizer, and the difference was not significant; In the flowering stage, the SPS activity at 120 d after flowering was the lowest, which was only higher than that in the control group; In the flowering stage, the activity of AI and NI was higher than that in other stages after the application of nitrogen fertilizer. In the new shoot growing stage, AI and NI activity in the late growth stage was lower than other treatments after supplement nitrogenous application, and the difference was not significant.4.Nitrogen fertilizer in the new shoot growth stage, the first stage of fruit expansion, the side shoot growth stage and the second stage of fruit expansion, the total nitrogen content of current year's new root at 120 d after flowering was significantly higher than that in the control group. Application of nitrogen fertilizer during the budding stage significantly increased the total nitrogen content of leaves and shoots in the early stage of growth; Nitrogen fertilizer application could significantly increase the contents of total nitrogen in leaves and shoots during the first stage of fruit expansion, the side shoot growth stage and the second expansion stage of fruit. The activity of NR was increased rapidly from 50 d to 90 d after flowering. The growth rate of nitrogen fertilizer treatment was the largest in the germination stage, the new shoot growth stage, flowering stage and the first stage of fruit expansion; During the germination stage, the new shoot growth stage, flowering stage and the first stage of fruit expansion, the leaf GDH activity at 20 d after flowering was significantly increased, which reached the highest value in the whole growth period; GS activity trends throughout the growing period showed a "M" shape. Application of nitrogen fertilizer during the germination period and new shoot growth stage significantly increased the GOGAT activity in the early stage of growth, and in the first stage of fruit enlargement, the changes of GOGAT activity during the whole growth period were changed.5.Sprouting period and new shoots flourishing period apply the nitrogen fertilizer increased the content of ZT, IAA, GA3 and ABA in the leaves; Nitrogen fertilizer significantly increased GA3 content in leaves at different stages, and changed the dynamic change of GA3 content during the whole growth period at different degrees; Sprouting period and new shoots flourishing period applied the nitrogen fertilizer, the content of(ZT+IAA+GA3)/ABA was significantly lower than the CK 5 d before the flowering; the content of(ZT+IAA+GA3)/ABA was higher than the CK in each period after 5d before the flowering.6.After applying nitrogen at flowering stage, soluble sugar, Vc, anthocyanin and tannin content in the fruit were higher, but the content of titration acid was the lowest at harvest time; The yield of grape in each treatment was as follows: nitrogen application at new shoot growing stage?germination stage=the first stage of fruit enlargement?flowering stage?secondary shoot growth stage?the second stage of fruit enlargement?the control. Although there was no significant difference in the yield of nitrogen fertilizer during the first stage of fruit enlargement and flowering stage, through the comprehensive analysis, the application of nitrogen fertilizer at flowering stage was benefit to improve the quality of fruit during harvest stage.