| Because of its high nutritional value and containing rich polyphenols,Lonicera caerulea gradually become popular in the market of functional food ingredients.It has antioxidant capacity,antitumor,anticancer,anti-inflammatory,inhibiting lipid peroxidation and platelet aggregation,protect the liver,prevent diabetes,lose weight,protect eyesight and other physiological functions of human beings.However the stability of phenolic compounds are easily influenced by the external environmental factors,such as pH,temperature,light intensity,metal ion,and so on.The main polyphenols extraction methods have many limitations which can not meet the requirements of polyphenols’ preservation,therefore,to find a more suitable polyphenols extraction method is particularly important.This experiment explores the influence of ultrahigh pressure extraction on the extraction rate of Lonicera caerulea antioxidant,antioxidant activity and cellular structure.Response surface was used to optimize the condition of ultrahigh pressure extraction of Lonicera caerulea anthocyanins and polyphenols.Comparison of the advantages and disadvantages of the two methods was made in the extraction of antioxidants of Lonicera caerulea.Conclusions are as follows:(1)Extraction pressure at 300MPa antioxidants extraction yield got the highest;Total phenol,anthocyanins and Vc content respectively got the highest at 300MPa,400 MPa;Antioxidant capacity got the highest level at 400 MPa.Electron microscopy images showed that ultrahigh pressure had huge damage to the cells of Lonicera caerulea,it’s helpful for the extraction of antioxidant and explained why antioxidants extraction rate was high through ultrahigh pressur extraction.According result of response surface the optimum conditions for polyphenol extraction were determined as follows:the extraction solvent was 50%ethanol with a solvent to solid ratio of 1:19(g/mL),and the extraction was performed at 30 ℃ with extraction pressure of 406 MPa for 11.5 min,the extraction yield of polyphenols was 776.21±1.43 mg/100 g;comparing with ultrasonic assisted extraction,antioxidant test results show that:polyphenol from ultrahigh pressure extraction showed significantly higher 2,2’-azino-bis(3-ehtylbenzothiazoline-6-sulfonic acid)diammonium salt radical(ABTS+·),Ferric Reducing Ability of Plasma(FRAP),1,1-diphenyl-2-picrylhydrazyl(DPPH)radical clearance rate than polyphenol from ultrasonic extraction and VC control group at same concentration levels.Although the ultrahigh pressure extraction need shorter time and polyphenols get higher antioxidant activity,but under the condition of extracting mass polyphenol and limited by the size of the container,ultrahigh pressure extraction’s efficiency and extraction yield was not as good as ultrasonic extraction.The response surface was used to optimize the UPE conditions for extracting anthocyanins from Lonicera caerulea berries.After optimization,the amount of extracted anthocyanins reached 26.57±0.55 mg/g,which was significantly higher than the mean TAC of extracts obtained using USE Furthermore,all three radical scavenging assays(DPPH,ABTS,and FRAP)indicated the UPE extracts had greater antioxidant activity than the USE extracts(P<0.05).In addition,HPLC-ESI-MS indicated that the composition and content of anthocyanins depended on the extraction method,that 12 anthocyanins were present in the Lonicera caerulea extracts from all two extraction methods,and that the UPE extracts contained the highest levels of total anthocyanins contend.Different extraction methods have great influence on the extraction amount of Lonicera caerulea anthocyanin monomer,extraction of ultrahigh pressure extraction is more conducive to the Lonicera caerulea anthocyanin... |
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