Studies On Extraction Of Phenols And Antioxidant Capacity Of Lonicera Caerulea L

In this dissertation, the optimal extraction processing of anthocyanin andnon-anthocyanins phenolic compouds from Lonicera Caerulea L. by organic solvent isstudied. Stability of anthocyanin also researched. Monomers of anthocyanin andnon-anthocyanins phenolic compouds are detected by HPLC and ability of antioxidation isanalyzed.In anthocyanin extraction tests, the sequence of extraction factors effected yield rate isextraction temperature, extraction time and solid-liquid ratio,ethanol concentration. Theoptimal extraction processing is as follows: the ratio of Lonicera Caerulea L. and70%alcohol was1to6, and the extraction processing undergone at40℃for80min to repeatedfor three times. By HPLC technology, Lonicera Caerulea L. had6.21mg/g3-glucoside-anthocyanin,1.33mg/g3,5-glucoside-dimethyl delphinidin,0.97mg/g3,5-glucoside-delphinidin and0.93mg/g3,5–glucoside-methyl delphinidin. In loniceracaerulea anthocyanins stability tests, light, high temperature, acid and peroxide can effectthe stability most seriously. Anthocyanins can be protected by appropriate Vc, butdecomposited if interaction for a long time. Fe3+and potassium sorbate can improve thestability of anthocyanins. Sucrose and xanthan gum have no effect on that. The optimalextraction processing is as follows: the ratio of90%ethyl acetate and Lonicera Caerulea L.is1to12and the processing is taken on for40min at90℃for two times. By HPLCtechnology, Lonicera Caerulea L. had4.81mg/g chlorogenic acid,1.79mg/g catechin,1.48mg/g quercetin and1.47mg/g ellagic acid. In polyphenol anti-oxidation tests, thescavenging activity of ABTS free radical reaches89%, that of hydroxyl free radical is91%, that of super oxygen anion is90%and that of DPPH free radical is70%. Polyphenolfrom Lonicera Caerulea L. has better reducing capacity.We concluded that the LoniceraCaerulea L possess strong antioxidance.