| Process water produced in extraction process of Camellia oleifera seed oil by aqueous enzymatic method was chosen as raw material in this study. The content of sasanqua saponin in process water was determined and the extracting technical conditions were optimizated. The crude product of sasanqua saponin was extracted by aqueous ethanol. Macroporous adsorptive resins with good adsorption and desorption properties were screened out and then applied on the purification of sasanqua saponin. Sasanqua saponin solution has certain surface activity, the parameters that affecting the surface activity and washing performance were determined. The main results were described as follows:1. The main compositions of oil-camellia seed process water were determined. The results showed that process water contained 5.76% sasanqua saponin,0.352% of total flavonoids,2.39% of crude protein,2.78% of total sugar, which showed that the process water were still valuable.2. Aqueous ethanol was used as extraction solvent to extract sasanqua saponin from wastewater of oil-camellia seed produced by aqueous enzymatic method. Three factors that are extract ethanol concentration, extraction temperature and pH value were used to do single factor test, to determinate the influence of ethanol concentration, extraction temperature and pH value in different degree on camellia saponin extraction rate. Response surface methodology was used to optimize the parameters obtained from extracting process of camellia saponin. The optimum condition is that the concentration of ethanol is 40% the extraction temperature is 60 ?, pH 4. Under this condition the extraction rate of sasanqua saponin is 67.18%?3. The adsorption and desorption properties of three kinds of macroporous adsorptive resins were compared. Among these resins adsorbing rate of AB-8 macroporous adsorptive resin was the fastest, also the desorbing rate was the highest under the same condition. Therefore, AB-8 macroporous adsorptive resin was chosen for the purification of sasanqua saponin. The single factor test condition were carried out to study the impact of AB-8 macroporous resin on purification of crude sasanqua saponin. Results showed that when the sasanqua saponin concentration was 25.00mg/mL, the adsorption time was 120min, the adsorption rate of sasanqua saponin was the best in the process of adsorption,. In desorption process, when the ethanol concentration was 70%, desorption time was 90 min, dose of desorbing liquid was 40.0mL for per gram resin, the purity of sasanqua saponin reached to 85.27% after purification.4. The parameters that affecting the surface activity of sasanqua saponin were determined, conclusions were as follows:a. When the temperature is 25?, mass fraction of sasanqua saponin is between 0.0012% and 0.050%, the surface tension of sasanqua saponin solution would decrease with the increase of the concentration of sasanqua saponin solution.b. When concentration of saponin solution is 0.1%, the contact angle of the solution is 41.5°lower than 90°, the contact angle decreases gradually with the increase of concentration of sasanqua saponin in solution, which means sasanqua saponin has good wetting properties.c. The solution foaming power of sasanqua saponin improved with the increase of the concentration. After 120min static standing sasanqua saponin solution bubble was still relatively stable, indicating that sasanqua saponin solution has strong foaming and foam stability.5. Commercial available strawberry tomato was selected as test material and washed by water as well as 5% sasanqua saponin solution respectively. By comparing the washing effect for some residual pesticide, results showed that the 5% camellia saponin solution could effective remove carbendazim from strawberry surface and 5% camellia saponin solution can remove dichlorvos, dimethoate, marla sulfur phosphorus at the removal rate of 38.44%, 18.93% and 30.68%, respectively. |
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