Mycorrhizal Fungi Diversity Of Nine Orchids In Liaoning Province

Orchids not only play an important role in the ecosystem,but also have extremely high ornamental value and medicinal value.Mycorrhizal fungi are crucial to the complement of orchids' life history.The study of orchid mycorrhizal fungi(OrMF)is of great value to the exploitation and protection of orchids.This study took 9 species of orchids,which grown in Liaoning,China,as examples to study the fungi communities in orchids' roots,rhizosphere soil and bulk soil using the next-generation sequencing technology.The results showed that: Amitostigma gracile,Platanthera chlorantha,Goodyera bomiensis and Oreorchis patens preferred to symbiotic with Ceratobasidiaceae.Cephalanthera longibracteata can be symbiotic with Thelephoraceae,Ceratobasidium and Sebacinaceae.Liparis makinoana preferred to symbiotic with Tulasnellaceae.Corallorhiza trifida was highly specific to OrMF and almost only symbiotic with Thelephoraceae.Spiranthes sinensis preferred to symbiotic with Rhizoctonia and Sebacinaceae.When studied the fungi communities,the results shown that there were obvious differences in the OrMF communities in the roots of the same orchids grown in either the same or different habitats.The different orchids grown in the same habitats had their own distinct OrMF community.Meanwhile,the OrMF communities in the roots of orchids and that in the soils also had obvious differences.The OrMF,which dominated in the roots of orchids,might just have a low abundance or even not existed in the soil around orchids.Then we analyzed the relationship between OrMF and the orchids' genetic relationship,and found that only the OrMF of S.sinensis and G.bomiensis showed a close relationship.Besides,there were a large number of non mycorrhizal fungi existed in orchids' roots,and the proportion of mycorrhizal fungi was far less than that of non mycorrhizal fungi,except for C.trifida.