The Potential Roles Of The LEA And DRRB Proteins In Early Embryo Development And In Response To Low Temperature And High Salinity In Artemia Sinica

Artemia sinica is a small crustacean animal living in high salt water.It is an important aquatic bait with strong resistance to stress,and an important experimental animal for molecular biology and developmental biology.Late embryogenesis abundant proteins(LEA)are stress resistance-related proteins that play crucial roles in protecting against desiccation,cold and high salinity in a variety of animals and plants.Developmental regulation RNA-binding protein(DRRB),involved in the regulation of biological growth and development in the cell,is closely related to the development and function of the nervous system.However,the expression pattern,distribution and functions of LEA proteins and DRRB proteins in the post-diapause period of Artemia sinica,and under high salinity and low temperature stresses,remain unknown.In this study,the complete cDNA sequences of the group 1 LEA(As-g1lea)?group 3LEA(As-g3lea)genes and As-drrb from A.sinica were cloned.The full-length cDNA of As-g1lea(GenBank Number: AMQ80946.1)was 960 bp,encoding a 182 amino acid protein,and As-g3lea(GenBank Number: AOV81545.1)was 2089 bp,encoding a 364 amino acid protein.The full-length cDNA of As-drrb(GenBank Number: AOV81546.1)was 1878 bp,encoding a 537 amino acid protein.As-g1 lea and As-g3 lea showed their highest expressions at0 h of embryonic development and both showed higher relative expression in embryonic,rather than adult,development stages.The abundances of As-G1 LEA,As-G3 LEA and trehalose were upregulated under low temperature and downregulated under high salinity stress.The expression of As-DRRB increased gradually during embryonic development and showed a stable expression at low temperature.The expression level of As-DRRB is decreased in high salt stress,which indicated that it could be related to early embryonic development of Artemia.These three genes had no tissue or organ specific expression.In this paper,we further analyzed the molecular mechanism of the poor environment of Artemia,which provided the scientific basis for the study of the molecular mechanism of the embryo regeneration of crustaceans and other invertebrates.