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Deep Underground Microbial Taxonomy Of New Species

Posted on:2016-06-12Degree:MasterType:Thesis
Country:ChinaCandidate:Y W TengFull Text:PDF
GTID:2310330491461614Subject:Chemical Engineering and Technology
Abstract/Summary:PDF Full Text Request
There are variety of unique microbial resources in Mohe permafrost regions. It is also the only high-latitude permafrost regions of China. Taxonomic studies of microorganisms in permafrost regions, not only enrich permafrost zone data on the microbial community and add new members to the microbial world, but also may help explore lurking gas hydrate in permafrost.Microbial taxonomy is an indispensable branche of microbiology, Which contains the results of microbiology. It is the most practical branche in microbiology. Three parts of bacterial taxonomy:classification, nomenclature and identification. Classification and identification of bacteria has the following parts:training features, morphological, physiological and biochemical characteristics, genetic characteristics, molecular biology characteristics.This study supply and improve the microbiological classification system. It also optimizes and supplies methods and techniques of microorganisms classification. The study works for identification and classification of two novel strains isolated from permafrost regions of Mohe. The taxonomic study of new species of bacteria aims at finding, understanding new species of bacteria and relationship with the known microorganisms.It can provide a theoretical basis for developing, utilizing, controlling and transforming microorganisms. It is necessary for subsequent functional studies and new metabolic products.Sphingobium belongs to Sphingomonadaceae. Members of Sphingomonadaceae mainly distribute in planktonic flora of freshwater and can be cultivated by oligo-culture. Sphingobium once was classified to the genus Sphingomonas by Yabuuchi.2001, with phylogenetic classification, chemical and physiological characteristics classification methods, Takeuchi divided Sphingomonas into four generas:strict sense Sphingomonas, Sphingolipid, Sphingobium and Sphingopyxis. Up to now, the genus Sphingobium contains 25 species. The type strain is Sphingobium yanoikuyae.Novel strains Z6T and Z7 are gram-negative, yellow colonies, aerobic and no sliding. According to the phenotype, genotype, chemical classification data, Z6T and Z7 are considered to belong to Sphingobium. The type strain is Z6T(= CGMCC 1.12891T=JCM 19983T). It was named Sphingobium sediminis.
Keywords/Search Tags:Mohe Basin, deep subsurface, microbial diversity, new species, polyphasic taxonomy
PDF Full Text Request
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