| Marine has broad distribution on earth, which contains a wealth of microbial resources. Marine microbes possess a strong environmental adaptability, and play an important role in the energy transfer and chemical cycles in marine environment. The study of marine microbes helps to further exploration in origin, evolution and adaption mechanism of microorganisms, and laid the foundation in developing and utilizing the marine resources. In this study, two classical habitat included deep-sea and offshore, was chosen for the research of microorganisms. We analyzed the diversity and community structure of the deep-sea sediment from Indian Ocean, and using the polyphasic taxonomic method to identified two strains isolated from seaweeds.Through the enrichment of cultivable bacteria from deep-sea sample, totally86strains was isolated. The strains belonged to4groups and16genus, in which two strains had low16S rRNA gene similarity and might to present novel species. Two archaeal libraries and two bacterial libraries was constructed by using the molecular biology method to clone16S rRNA gene. The archaeal libraries contained195clones, and there were27OTUs in library A1and47OTUs in library A5. They belonged to Crenarchaeota and Euryarchaeota. The bacterial libraries got530clones, library B1had132OTUs and B5had101OTUs. The dominant groups of two samples were Proteobacteria, Planctomycetes and Chloroflexi.The identification of two strains isolated from seaweeds which collected from Zhoushan sea area was based on the polyphasic taxonomy. According to the phenotype, physiological and biochemical characterization, chemotaxonomic characterization and analysis of16S rRNA gene sequence, srtain8-1bT was proposed to represent a novel species in Flavobacteriaceae(Aequorivita viscosa) and strain CF17T to represent a novel species belonged to Alphaproteobacteria (Gemmobacter megaterium). The results enriched the microbial resources of East China Sea. |
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