The Fuction Of PGRP-SC2 In The Immune Response Of Artemia Sinica

Artemia sinica,which belongs to Phylum Arthropoda,Class Crustacea,Subclass Branchiopoda,Order Anostaca,Family Artemiidae,Genus Artemia,is a small aquatic crustacean,lives in the hyperosmotic environment of salt lakes in China.It has been used economically as a main food resource for newly bornfish and crustacean species in aquaculture because of its high protein and unsaturated fatty acid content in the nauplii.Its resistance to high salinity,drying,low temperature,pressure and other adverse environments stress led to Artemia sinica being widely used in various fields,ranging from developmental biology to evolution and ecology.It has received increasing attention from scientists especially innate immune research,where it is widely studied as an animal model.Peptidoglycan-recognition protein-SC2 precursor-like protein(PGRP-SC2)is a significant factor of innate immunity in Artemia sinica.It plays an important role in the immune response to Gram bacteria of Artemia sinica.In our study,a 678 bp full-length c DNA of pgrp-sc2 was obtained with an open reading frame of 558 bp contained 185 amino acids and had a calculated molecular mass of 19.6 k Da.The predicted protein contains a PGRP and a Amidase2 domain which means PGRP-SC2 is a member of PGRP family and has N-acetylmuramoyl-L-alanine amidase activity.Real-time quantitative PCR,Western blotting and ISH were used to determine the transcription,translation expression level and location of PGRP-SC2 during embryonic development and bacterial stimulation.During the embryonic development of A.sinica,the 0h and 5h stages are the the highest expression level stages of pgrp-sc2.Under challenge by Gram bacteria,the transcription and translation expression level of pgrp-sc2 increased as the bacteria concentration increased.